[KE peptide regulates SIRT1, PARP1, PARP2 gene expression and protein synthesis in human mesenchymal stem cells aging.].
Khavinson. V K VK; Linkova. N S NS; Ashapkin. V V VV; Shilovsky. G A GA; Borushko. N V NV; Petukhov. M G MG; Vanuyshin. B F BF
Key Findings
- KE peptide increased SIRT1 gene expression ~6‑fold and protein levels ~8‑fold in young MSCs
- KE peptide reduced PARP1 gene expression 2.1‑fold and protein synthesis 5.3‑fold during MSC aging
- KE peptide reduced PARP2 gene expression 2.1‑fold and protein synthesis 4.7‑fold during MSC aging
- Molecular modeling suggests KE peptide binds promoter DNA sequences of SIRT1, PARP1, and PARP2
Practical Outcomes
- At this stage the findings are interesting for biohackers but not ready for direct supplementation. More animal and human studies are needed to determine safe doses and real‑world effects. Until then, consider the peptide as a research candidate rather than a proven anti‑aging supplement.
Summary
The KE peptide (Lys‑Glu, also called vilon) was shown in lab-grown human stem cells to boost a longevity‑related protein called SIRT1 and lower two DNA‑repair proteins (PARP1 and PARP2) that tend to rise as cells age. The peptide appears to bind specific DNA sequences in the gene promoters, which may explain how it changes gene activity. These results suggest a possible anti‑aging effect, but they are limited to cell‑culture experiments and don’t tell us how to use the peptide in people.
Abstract
It was shown that KE peptide (Lys-Glu, vilon) has immunomodulatory, oncostatic and geroprotective effects. The aim of this work is to evaluate the effect of the KE peptide on gene expression and protein synthesis of SIRT1, PARP1, PARP2 during aging of human mesenchymal stem cells (MSC). The KE peptide increased gene expression and synthesis of the SIRT1 protein in «young» MSCs by 6 and 8,2 times, respectively. The KE peptide reduced gene expression and PARP1 protein synthesis during MSC aging by 2,1 and 5,3 times, respectively; and also reduced gene expression and PARP2 protein synthesis by 2,1 and 4,7 times, respectively. According to molecular modeling data, the KE peptide can interact with the GCGG sequence of double-stranded DNA (dsDNA) in the classical B-form and with the GGGC sequence of the curved dsDNA nucleosome. The indicated dsDNA sequences were found in the promoters of the human SIRT1, PARP1, PARP2 genes. Thus, the KE peptide regulates gene expression and synthesis of SIRT1, PARP1, PARP2 proteins in human mesenchymal stem cells during replicative ageing, which underlies the biological activity and geroprotective effect of this peptide.
Study Information
pubmed
2023