Pentadecapeptide BPC 157 enhances the growth hormone receptor expression in tendon fibroblasts.
Chang. Chung-Hsun CH; Tsai. Wen-Chung WC; Hsu. Ya-Hui YH; Pang. Jong-Hwei Su JH
Key Findings
- BPC‑157 significantly raises growth‑hormone receptor (GHR) mRNA and protein levels in rat Achilles‑tendon fibroblasts.
- Higher GHR levels make the cells more responsive to added growth hormone, boosting cell proliferation and PCNA expression.
- The GHR‑JAK2 signaling pathway is activated in a time‑dependent manner when BPC‑157‑treated cells receive growth hormone.
Practical Outcomes
- For biohackers interested in tendon repair, BPC‑157 may enhance the tissue’s sensitivity to growth hormone, potentially accelerating healing. However, the data are from isolated rat cells, so human dosing, safety, and real‑world effectiveness remain unproven. Use it cautiously, and consider it as a supportive agent rather than a standalone solution until more clinical evidence emerges.
Summary
In rats, the peptide BPC‑157 makes tendon cells produce more growth‑hormone receptors, which lets the cells respond stronger to growth hormone and multiply faster. This effect grows with higher doses and longer exposure, suggesting a possible way BPC‑157 helps heal tendons.
Abstract
BPC 157, a pentadecapeptide derived from human gastric juice, has been demonstrated to promote the healing of different tissues, including skin, muscle, bone, ligament and tendon in many animal studies. However, the underlying mechanism has not been fully clarified. The present study aimed to explore the effect of BPC 157 on tendon fibroblasts isolated from Achilles tendon of male Sprague-Dawley rat. From the result of cDNA microarray analysis, growth hormone receptor was revealed as one of the most abundantly up-regulated genes in tendon fibroblasts by BPC 157. BPC 157 dose- and time-dependently increased the expression of growth hormone receptor in tendon fibroblasts at both the mRNA and protein levels as measured by RT/real-time PCR and Western blot, respectively. The addition of growth hormone to BPC 157-treated tendon fibroblasts dose- and time-dependently increased the cell proliferation as determined by MTT assay and PCNA expression by RT/real-time PCR. Janus kinase 2, the downstream signal pathway of growth hormone receptor, was activated time-dependently by stimulating the BPC 157-treated tendon fibroblasts with growth hormone. In conclusion, the BPC 157-induced increase of growth hormone receptor in tendon fibroblasts may potentiate the proliferation-promoting effect of growth hormone and contribute to the healing of tendon.
Study Information
pubmed
2014
2014-11-19T00:00:00.000Z
10.3390/molecules191119066
84
39