[The effect of the amino acids and cardiogen on the development of myocard tissue culture from young and old rats].
Chalisova. N I NI; Lesniak. V V VV; Balykina. N A NA; Urt'eva. S A SA; Urt'eva. T A TA; Sukhonos. Iu A IuA; Zhekalov. A N AN
Key Findings
- Cardiogen at 10ā»Ā¹Ā²āÆM strongly increased cell proliferation in heart tissue from both 3āmonthāold and 24āmonthāold rats.
- Cardiogen reduced the expression of the p53 protein, indicating it may block the cellādeath (apoptosis) pathway.
- Only a few of the 20 tested amino acids boosted cell growth, and they were effective mainly in tissue from young rats.
Practical Outcomes
- The study shows cardiogen has promising antiāaging effects on heart cells in rats, but itās an earlyāstage, ināvitro experiment. Thereās no human dosage, safety, or efficacy data, so it isnāt ready for any DIY or clinical protocol yet. It simply points to a potential heartāprotective peptide that needs far more research before practical use.
Summary
A tiny amount of the synthetic peptide called cardiogen helped heart cells from both young and old rats grow and avoid death in a lab dish, while most regular amino acids only helped the young rat cells.
Abstract
The effect of 20 amino acids and the synthetic tetrapeptide cardiogen in concentration 10(-12) M was investigated in organotypic tissue culture on the cell proliferation and apoptosis development in myocard tissue explant in 3- and 24-months old rats. 7 of 20 amino acids stimulated the cell proliferation in the young rats. In the same time, only 2 amino acids were active in myocard culture from the old rats. The tetrapeptide cardiogen demonstrated the great stimulating effect on the proliferation both in tissues from young and old rats. The immunohistochemical study demonstrated a decrease of the p53 protein expression by cardiogen action. This fact can testify that cardiogen inhibits the apoptosis process in the myocard tissue.
Study Information
pubmed
2009