[Studies on the isolation of endogenous sleep factors from Tupaia Belangeri Chinensis (TBC) after sleep deprivation].
Liu. S Y SY; Zhang. W Y WY; Li. H Y HY; Dai. X J XJ; Zhang. Y Y; Xu. J C JC; Chen. L L LL; Wang. S Q SQ; Qian. C H CH
Key Findings
- Two urine‑derived fractions (S2C and S4B) enhanced delta‑wave sleep in rabbits after intracerebroventricular injection.
- The amino‑acid composition of these fractions differs from known sleep factors like DSIP.
- Further purification steps (Sephadex G‑25 and LH‑20) retained the sleep‑enhancing activity.
Practical Outcomes
- The study is an early‑stage animal experiment and does not provide a usable protocol, dosage, or safety data for humans. For biohackers, it simply signals that there may be undiscovered natural sleep‑enhancing compounds, but no actionable steps can be taken yet.
Summary
Researchers isolated new sleep‑promoting substances from the urine of sleep‑deprived tree shrews and showed that two purified fractions (called S2C and S4B) increased deep (delta) sleep when injected directly into the brains of rabbits. These substances are chemically different from the well‑known sleep peptide DSIP.
Abstract
The present study was undertaken to explore endogenous sleep factors isolated from 48-72 h sleep deprived (SD) male Tupaia belangeri chinensis (TBC). Only drink ad libitum (10% glucose) was available within 24 h before collection of urine. Controlled "clean" urinary samples were pooled and stored at 20 C. Fraction I-V from the urine were determined after ultrafiltration and Sephadex-G15. Amino-acid analysis of each fraction was automatically done by a 835 Amino-acid Analyzer, respectively. Bioassay was performed in 40 adult rabbits weighing 2.5-3.5 kg of either sex. Experiments were undertaken via the mesodiencephalic intraventricular infusion. Results show that S2C (Fraction-III) (50 micrograms/rabbit, i.c.v.) exhibited significant delta-enhancing effect compared to the controls. Further purification was done with Sephadex G-25 and Sephadex LH-20. The more purified S4B (50 micrograms/rabbit, i.c.v.) also exhibited significant delta-enhancing effect compared to the controls. The amino-acid analysis of Fraction-III revealed that the compositional contents of S2C and S4B are different from what have been known with Factor S, DSIP and SPS.
Study Information
pubmed
1994