Elucidation of the mechanisms of the DSIP effect on the CNS functional state is one of the leading problems of neuroendocrinology. The investigation of DSIP physiological properties as antistress factor in systemic mechanisms of emotional stress is of great interest. The purpose of our studies was to investigate the character of changes in intercentral interrelationships among hypothalamus, limbic system and reticular formation in augmentation of corticosteroids level up to the stressful one. We also studied the character of changes in intercentral interrelationship under the DSIP effect. The latter depends on the initial level of corticosteroids in the blood and is more obvious at the stress level. DSIP, ACTH and steroid hormones facilitate the process of the self-organization of the brain as well as the formation of the current behavioral responses.