Morphochemical study of hippocampus of august rats after systemic treatment with amphetamine followed by injection of delta sleep-inducing peptide.
Gershtein. L M LM; Sergutina. A V AV; Rakhmanova. V I VI
Key Findings
- Chronic amphetamine (2.5 mg/kg/day for 3 weeks) increased the size of neuronal cytoplasm and nuclei in hippocampal CA3 neurons.
- Protein content and concentration in these neurons also rose after amphetamine treatment.
- A single injection of DSIP (60 µg/kg) did not alter or reduce the amphetamine‑induced changes.
Practical Outcomes
- For biohackers, this study indicates that DSIP is unlikely to counteract or repair amphetamine‑related brain changes, so it should not be relied on for neuro‑protective purposes after stimulant use. No dosage adjustments or new protocols involving DSIP are supported by these findings.
Summary
In rats that were given amphetamine for three weeks, brain cells in a part of the hippocampus got bigger and packed with more protein. Giving a single dose of delta sleep‑inducing peptide (DSIP) after the amphetamine did not change these effects – the brain cells stayed enlarged. This suggests DSIP does not reverse or protect against the structural changes caused by chronic amphetamine in this animal model.
Abstract
Quantitative interferometry showed that chronic amphetamine administration to August rats (2.5 mg/kg/day for 3 weeks) increased the area of neuronal cytoplasm and nuclei and content and concentrations of proteins in hippocampal CA3 neurons. These changes persisted after single injection of delta sleep-inducing peptide (60 microg/kg). The reaction of the entire neuronal population of hippocampal CA3 neurons to amphetamine is similar.
Study Information
pubmed
2006
2006-04-01T00:00:00.000Z
10.1007/s10517-006-0201-5
6