Human pheochromocytoma cells studied in culture contain large amounts of DSIP-like material.
Nilsson. O O; Wängberg. B B; Wigander. A A; Lundmark. K K; Dahlström. A A; Ahlman. H H; Bjartell. A A; Ekman. R R
Key Findings
- DSIP‑like material is present in all nine human pheochromocytoma tumor samples examined.
- The peptide is stored in the dense core of secretory granules inside the tumor cells.
- Cultured tumor cells release DSIP‑like material into the culture medium, and the pattern of release varies between tumors.
Practical Outcomes
- For biohackers, this study mainly shows that DSIP is produced by a specific type of tumor cell, but it doesn't provide any dosage, safety, or performance data for supplementation. It confirms DSIP is an endogenous peptide, yet offers no direct guidance for real‑world use.
Summary
Researchers found that a peptide called delta sleep‑inducing peptide (DSIP) or something very similar is naturally made and stored inside certain tumor cells from the adrenal gland, and these cells can release it into their surroundings.
Abstract
Delta sleep-inducing peptide (DSIP)-like immunoreactive (LI) material has been detected in nine different human pheochromocytoma tumors by immunocytochemistry. In primary tumors subjected to indirect immunofluorescence a variable number of tumor cells (25-75%) showed positive cytoplasmic labeling after incubation with DSIP antiserum. Tumor cells grown in culture were strongly labeled by the DSIP antiserum with DSIP-LI concentrated to cell bodies. Electron microscopic immunocytochemistry (immunogold labeling) of pheochromocytoma cells demonstrated DSIP-LI over the dense core of secretory granules. The presence of DSIP-LI in several HPLC fractions from conditioned culture media indicates secretion of DSIP-LI from cultured pheochromocytoma cells. The observations suggest that DSIP-LI is synthesized and stored in secretory granules before release. The different HPLC profiles from each of the tumors may reflect differences in processing or turnover of DSIP-LI in pheochromocytoma cells.
Study Information
pubmed
1991
10.1016/0196-9781(91)90063-u