Sleep-promoting effect following intracerebroventricular injection of a phosphorylated analogue of delta sleep-inducing peptide (DSIP-P) in rats.
Nakagaki. K K; Ebihara. S S; Usui. S S; Honda. Y Y; Takahashi. Y Y
Key Findings
- Intracerebroventricular injection of DSIP‑P (20‑200 pmol/kg) boosted slow‑wave sleep (SWS) by 17.3% and paradoxical (REM) sleep by 32.3% in rats.
- The sleep‑enhancing effect persisted into the following light period and returned to baseline after two days.
- Higher dose (200 pmol/kg) did not shorten sleep latency, indicating the peptide improves sleep depth rather than onset.
Practical Outcomes
- For biohackers, the study shows that DSIP can influence sleep architecture, but the delivery method (direct brain injection) is not feasible outside a lab. It suggests that DSIP or its analogues might have sleep‑enhancing potential, but more research is needed to develop safe, peripheral administration routes for humans.
Summary
In rats, a chemically modified version of delta sleep‑inducing peptide (DSIP‑P) given directly into the brain increased deep (slow‑wave) sleep by about 17% and REM sleep by about 32% during the night, with effects lasting into the next day, but it required a brain injection and did not change how quickly the rats fell asleep.
Abstract
The effect of phosphorylated delta sleep-inducing peptide (DSIP-P) on sleep of rats was studied. DSIP-P (20 or 200 pmol/kg) was injected into the third cerebroventricle of male rats immediately before the onset of the dark period of a 12:12 h light-dark cycle. DSIP-P resulted in increases of slow-wave sleep (SWS) (17.3%, P less than 0.01) and paradoxical sleep (PS) (32.3%, P less than 0.05) during the subsequent dark period without shortening sleep latency in the dose of 200 pmol/kg. The SWS-promoting effect was carried over to the next light period. These changes returned to control levels on the second day. These results indicate that DSIP-P is a long-lasting sleep-promoting substance in rats.
Study Information
pubmed
1988
1988-08-31T00:00:00.000Z
10.1016/0304-3940(88)90761-6
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