The effect of delta sleep-inducing peptide (DSIP) and phosphorylated DSIP (P-DSIP) on the apomorphine-induced hypothermia in rats.
Tsunashima. K K; Masui. A A; Kato. N N
Key Findings
- DSIP and P‑DSIP both boost apomorphine‑induced hypothermia in rats.
- P‑DSIP’s effect appears and disappears more quickly than DSIP’s.
- Effective doses are very low (10 ng–1 µg) with an inverted bell‑shaped dose‑response curve.
Practical Outcomes
- The data hint that DSIP influences dopamine‑related thermoregulation, but it’s an animal study with no direct human protocol. While it suggests ultra‑low doses might be biologically active, more research is needed before applying this to human sleep, metabolism, or performance regimens.
Summary
In rats, both the natural delta sleep‑inducing peptide (DSIP) and a phosphorylated version (P‑DSIP) make a dopamine drug cause a bigger drop in body temperature. The effect shows up at extremely low doses (as little as 10 ng) and peaks around 1 µg, with the phosphorylated form acting faster but wearing off sooner. Blocking dopamine receptors stops the effect, suggesting DSIP works through the dopamine system.
Abstract
Delta sleep-inducing peptide (DSIP) and P-DSIP, phosphorylated analogue, were found to have enhancing effects on hypothermia induced by i.p. injection of apomorphine (2 mg/kg), a dopamine agonist. Further, the action of P-DSIP appeared and diminished more quickly than that of DSIP. A minimal effective dose of these peptides was 10 ng and the dose-response relationship exhibited an inverted bell-shape with a maximal effective dose of 1 microgram. By the pretreatment of anti-DSIP the enhancing effect of DSIP but not P-DSIP, was totally abolished and the action of both peptides was antagonized by haloperidol. These findings suggest that DSIP and P-DSIP have a close relation to the dopaminergic system on the thermoregulatory mechanisms.
Study Information
pubmed
1990
1990-02-26T00:00:00.000Z
10.1016/0006-8993(90)90748-z