Immunoreactive delta sleep-inducing peptide in pituitary adrenocorticotropin/alpha-melanotropin cells and adrenal medullary cells of the pig.
Ekman. R R; Bjartell. A A; Ekblad. E E; Sundler. F F
Key Findings
- DSIP‑like immunoreactivity is present in a subset of pig pituitary cells that store adrenocorticotropin (ACTH) and alpha‑melanotropin.
- The same DSIP‑like material is found in adrenal medulla cells that store noradrenaline.
- Chromatography shows the endogenous DSIP‑like peptide does not co‑elute with synthetic DSIP, implying it may be an N‑terminally truncated fragment.
- The anterior/intermediate pituitary lobes and adrenal medulla are suggested as sources of DSIP‑like peptides.
Practical Outcomes
- For biohackers, this study mainly adds basic knowledge about where DSIP‑like peptides are produced in the body. It does not provide dosing guidelines, efficacy data, or direct ways to use DSIP for performance or longevity. Until functional effects of these endogenous fragments are clarified, the findings have limited immediate application.
Summary
Researchers found that a peptide called delta sleep‑inducing peptide (DSIP) or DSIP‑like fragments are naturally present in pig pituitary cells that make ACTH and alpha‑melanotropin, and in adrenal medulla cells that store noradrenaline. The material they detected didn't match the synthetic version of DSIP, suggesting it may be a shortened form of the peptide. This points to the pituitary and adrenal glands as possible sources of DSIP‑like peptides in the body.
Abstract
Immunoreactive delta sleep-inducing peptide (DSIP) is known to occur in the central nervous system and in body fluids including cerebrospinal fluid, blood and urine. However, the exact nature of the immunoreactive material demonstrated has been a matter of discussion. In the present study, DSIP-like immunoreactivity was demonstrated in the porcine pituitary and adrenal medulla using radioimmunoassay and immunocytochemistry. In the pituitary, the DSIP-like material was present in a subpopulation of the cells storing adrenocorticotropin and alpha-melanotropin. The DSIP immunoreactive cells in the adrenal medulla were identical with a major population of the noradrenaline-storing cells. High-performance liquid chromatography of tissue extracts revealed one major peak of DSIP-like immunoreactivity which did not coelute with synthetic DSIP. The immunoreactive material may represent N-terminally truncated fragments of DSIP. The present results suggest that the anterior and intermediate lobes of the pituitary and the adrenal medulla are potential sources of DSIP-like peptides.
Study Information
pubmed
1987
1987-04-01T00:00:00.000Z
10.1159/000124744
19