Interacting sleep-modulatory effects of simultaneously administered delta-sleep-inducing peptide, muramyl dipeptide and uridine in unrestrained rats.
Kimura. M M; Honda. K K; Komoda. Y Y; Inoué. S S
Key Findings
- Combining DSIP with muramyl dipeptide increased deep sleep only in the middle of the infusion period.
- DSIP plus uridine gave a modest deep‑sleep boost later in the infusion, with little effect on REM.
- Muramyl dipeptide plus uridine raised both deep and REM sleep early to mid‑infusion.
- All three together produced a large early‑mid increase in deep sleep and an early rise in REM sleep.
Practical Outcomes
- These results hint that DSIP might work better when paired with other compounds, but the study used invasive brain infusions in rats, so it can't be directly applied to human sleep hacks. For now, biohackers should treat this as basic science that needs human trials before any dosage or protocol changes.
Summary
In rats, giving a mix of three brain‑injected substances—DSIP, muramyl dipeptide, and uridine—changed sleep patterns in ways that depended on which ones were combined. Different pairings boosted deep sleep (SWS) or REM sleep at different times, and using all three together caused a big jump in deep sleep early on and a rise in REM sleep right at the start.
Abstract
A 10-h nocturnal intracerebroventricular infusion of 2 or 3 sleep substances such as 2.5 nmol delta-sleep-inducing peptide (DSIP), 2.0 nmol muramyl dipeptide (MDP) and 10 pmol uridine resulted in a significant, combination-dependent change in sleep-waking dynamics, which was quite different from the time-course sleep promotion induced by the single administration of each substance. The DSIP-MDP combination was characterized by a profound increase in SWS only at the middle infusion period. PS was little affected. The DSIP-uridine combination exerted a slight increase in SWS at the late infusion period with little change in PS parameters. The MDP-uridine combination caused a marked increase in both SWS and PS only at the early to middle infusion period. The DSIP-MDP-uridine combination induced an extremely large increase in SWS at the early to middle phase of the infusion period. PS significantly increased only at the early phase. These results may suggest that the sleep substances acted in tandem, either synergistically or antagonistically, on the sleep regulatory mechanism.
Study Information
pubmed
1987
1987-12-01T00:00:00.000Z
10.1016/0168-0102(87)90031-9
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