DSIP reduces amphetamine-induced hyperthermia in mice.
Graf. M V MV; Kastin. A J AJ; Coy. D H DH; Zadina. J E JE
Key Findings
- DSIP reduces amphetamine‑induced hyperthermia in mice at 0.1 nmol/kg and 150 nmol/kg doses.
- The D‑Ala4‑DSIP analog lowers temperature rise at 50‑150 nmol/kg, showing a bell‑shaped dose‑response.
- DSIP‑P (phosphorylated analog) and DSIP at other doses do not significantly affect temperature, and the effect is absent in rats.
Practical Outcomes
- For biohackers experimenting with DSIP, the data suggest that only very narrow dose windows may impact temperature regulation under stimulant stress, and results may not translate to humans or other species. The findings are not directly actionable for general health, longevity, or performance protocols, but they highlight the importance of precise dosing and the potential for complex dose‑response curves when using peptide analogs.
Summary
In mice, the sleep‑related peptide DSIP can lower the body‑temperature spike caused by a high dose of amphetamine, but only at very specific low (0.1 nmol/kg) and high (150 nmol/kg) doses. A modified version of the peptide (D‑Ala4‑DSIP) works in a middle dose range, while another analog (DSIP‑P) does not help. The effect is not seen in rats and disappears at the strongest amphetamine dose.
Abstract
The effects of delta sleep-inducing peptide (DSIP) have not been fully determined. Besides sleep-inducing activities, effects on locomotor behavior, stress-reduction, and temperature-regulation have been published. It was reported that DSIP reversed the increase in temperature of rats injected at room temperature with 15 mg d-amphetamine per kg body weight. We examined this effect in mice with 9 different concentrations of DSIP in addition to D-Ala4-DSIP and an analog, DSIP-P, phosphorylated at the serine in position 7. A reduction of the increased temperature was observed in mice but not in rats. This effect was only significant after pretreatment with 0.1 and 150 nmol DSIP/kg, but not the other doses. D-Ala4-DSIP decreased the rise in temperature between 50-150 nmol/kg, but DSIP-P showed no such effect nor was DSIP able to significantly reduce the increase of temperature induced by the larger dose of 30 mg/kg d-amphetamine. A bell-shaped dose-response curve was found for D-Ala4-DSIP; for DSIP two active dose ranges were observed. Thus, complex dose-effect relationships seem to exist for DSIP (and perhaps its analogs) in thermoregulation.
Study Information
pubmed
1984
1984-08-01T00:00:00.000Z
10.1016/0031-9384(84)90114-8
16
16