Effects of intracerebroventricular injection of delta sleep-inducing peptide (DSIP) and an analogue on sleep and brain temperature in rats at night.
Obál. F F; Török. A A; Alföldi. P P; Sáry. G G; Hajós. M M; Penke. B B
Key Findings
- A single intracerebroventricular (ICV) dose of DSIP (7 nmol/kg) did not increase total sleep time in rats.
- Both DSIP and its analogue C‑DSIP caused a small rise in wakefulness 6‑9 hours after injection.
- Brain temperature patterns after DSIP or C‑DSIP were indistinguishable from control (artificial CSF) injections.
Practical Outcomes
- For biohackers looking to use DSIP as a sleep aid, this study suggests that a one‑time brain injection at night is unlikely to help and may even increase wakefulness later. Since the method (ICV injection) isn’t practical for humans, the findings have limited direct application, but they caution against expecting strong sleep‑promoting effects from DSIP alone.
Summary
Injecting delta sleep‑inducing peptide (DSIP) directly into the brain of rats at the start of their night did not make them sleep more. In fact, it caused a modest increase in wakefulness a few hours later, and it didn’t change brain temperature.
Abstract
The effects of ICV injections of DSIP and omega-amino-caprilyl-DSIP (C-DSIP) on the sleep-wake activity and brain temperature (Tbr were studied in rats. The substances (7 nmol/kg) were injected at dark onset, and the sleep-wake activity and Tbr were recorded for 24 hr (dark and light periods, 12 hr each). Relative to the control recordings obtained after artificial CSF injection, the duration of sleep did not increase after either DSIP or C-DSIP. The only significant reaction was an increase of W 6 to 9 hr after the injection of either peptide. The course of Tbr after DSIP and C-DSIP was also identical to that recorded after the injection of artificial CSF. It seems that DSIP administered in a single ICV injection at dark onset does not promote sleep. The increase in W might be attributed to an indirect effect of DSIP or to a degradation product of the peptide.
Study Information
pubmed
1985
1985-12-01T00:00:00.000Z
10.1016/0091-3057(85)90099-1
12
29