Radioimmunoassay of delta sleep-inducing peptide using an iodinated p-hydroxyphenylpropionic acid derivative as tracer.
Ekman. R R; Larsson. I I; Malmquist. M M; Thorell. J I JI
Key Findings
- A radioimmunoassay for DSIP was successfully developed.
- The assay uses an iodinated p‑hydroxyphenylpropionic acid derivative as the tracer.
- The detection limit is extremely low (0.1 ng/ml, or 10 pg per test tube).
Practical Outcomes
- The new test lets researchers accurately measure DSIP levels in body fluids, which could support future studies on its role in sleep and performance. However, it does not provide dosing guidelines, safety data, or direct performance benefits for biohackers.
Summary
Scientists created a very sensitive lab test that can detect tiny amounts of the delta sleep‑inducing peptide (DSIP) in blood or other fluids. The test uses a special radioactive tag and can pick up as little as 0.1 ng/ml of the peptide.
Abstract
A highly sensitive radioimmunoassay for delta sleep-inducing peptide (DSIP) has been developed. A p-hydroxyphenylpropionic acid conjugate of DSIP was used for radioiodination. Using reversed-phase high performance liquid chromatography the labelled DSIP derivative was isolated in a high yield and with a high specific activity. The assay allows measurement of DSIP-like material in body fluids with a minimum detectable concentration of 0.1 ng/ml standard DSIP (10 pg/tube).
Study Information
pubmed
1983
1983-08-01T00:00:00.000Z
10.1016/0167-0115(83)90266-5
33
9