Evidence that [125I]N-Tyr-delta sleep-inducing peptide crosses the blood-brain barrier by a non-competitive mechanism.
Banks. W A WA; Kastin. A J AJ; Coy. D H DH
Key Findings
- DSIP crosses the rat blood‑brain barrier and the dog blood‑CSF barrier.
- The crossing occurs via a non‑competitive mechanism—extra DSIP or analogs don’t stop it.
- Intact radioactive DSIP was found in brain tissue, confirming it isn’t broken down before crossing.
Practical Outcomes
- For DIY health enthusiasts, this means that peripheral (e.g., subcutaneous or intravenous) DSIP can reach the brain, supporting its use for central effects like sleep modulation. However, the study doesn’t provide dosing, safety, or efficacy guidance, so any protocol should be approached cautiously and supplemented with further evidence.
Summary
Scientists showed that the sleep‑inducing peptide DSIP can move from the bloodstream into the brain in rats and into the cerebrospinal fluid in dogs, and this movement isn’t blocked by adding extra DSIP or similar molecules.
Abstract
Delta sleep-inducing peptide (DSIP), a nonapeptide, has previously been shown to cross the blood-brain barrier (BBB) in rats and the blood-CSF barrier in dogs. New experiments were conducted to determine if this crossing was competitive. Neither DSIP nor several analogs, including non-radioactive [127I]N-Tyr-DSIP, injected by the jugular vein or carotid artery, inhibited passage of radioactive [125I]N-Tyr-DSIP across the rat BBB. Column chromatography of brain samples confirmed that the radioactivity in the brain represented intact [125I]N-Tyr-DSIP and that the non-radioactive competing materials did not interfere with the degradation or binding of [125I]N-Tyr-DSIP. In addition, N-Tyr-DSIP was unable to inhibit the appearance of radioactive [125I]N-Tyr-DSIP in the CSF of dogs. In conclusion, the evidence from these experiments suggests that [125I]N-Tyr-DSIP crosses the rat BBB and dog blood-CSF barrier by a non-competitive mechanism.
Study Information
pubmed
1984
1984-06-03T00:00:00.000Z
10.1016/0006-8993(84)91088-6
61
14