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DSIP

Emideltide, DSIP nonapeptide, Delta sleep-inducing peptide

Quick Stats
Studies 458
Trials 82
Overview Studies Clinical Trials
Score 1
1978 pubmed 57 citations

Radioimmunoassay of DSIP-like material in rat brain.

Kastin. A J AJ; Nissen. C C; Schally. A V AV; Coy. D H DH

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Key Findings

  • A radioimmunoassay for DSIP was successfully developed using a synthetic version of the peptide.
  • DSIPā€‘like immunoreactivity was detected throughout rat brain tissue, with the highest levels in the thalamus (ā‰ˆ12ā€Æpg/mg).
  • The assay may also pick up similar proteins, so results should be interpreted cautiously.

Practical Outcomes

  • For biohackers, this study mainly shows that DSIP exists naturally in the brain and that measuring it is technically possible, but it doesn't provide dosing guidelines or clear health benefits. It suggests DSIP might have roles beyond sleep, yet more research is needed before practical protocols can be designed.

Summary

Researchers created a test to measure a sleepā€‘related peptide called DSIP in rat brains and found small amounts of DSIPā€‘like material, especially in the thalamus. The test works, but because DSIP shares pieces with other proteins, we can't be sure exactly what's being measured.

Abstract

Antibodies generated in a rabbit by immunization with synthetic delta-sleep inducing peptide (DSIP) showed no cross-reactivity with 19 naturally-occurring peptides or analogues and were used to establish a radioimmunoassay. Since DSIP is not readily iodinated by conventional methods, N-Tyr-DSIP was synthesized to prepare the tracer; the dose-response curve for N-Tyr-DSIP was exactly parallel to that for DSIP. With this assay, DSIP-like immunoreactivity was detected in the brain of rats. The highest brain values were found in the thalamus (11.9 +/- 1.3 pg/mg). The widespread presence of DSIP-like material throughout the body as well as the shared amino acid sequences of DSIP with other proteins suggest caution in defining the material being measured by this new assay. Nevertheless, the demonstration of DSIP-like activity in brain tissue and elsewhere warrants consideration of functions in addition to a possible role in sleep.

Study Information

Provider

pubmed

Year

1978

Date

1978-11-01T00:00:00.000Z

DOI

10.1016/0361-9230(78)90019-9

Citations

57

References

16