In Vitro and in Vivo Studies of pH-Sensitive GHK-Cu-Incorporated Polyaspartic and Polyacrylic Acid Superabsorbent Polymer.
Sharma. Shilpa S; Anwar. Mohammad Faiyaz MF; Dinda. Amit A; Singhal. Maneesh M; Malik. Amita A
Key Findings
- The polymer is blood‑compatible (low protein adsorption and normal clotting).
- GHK‑Cu is released from the polymer in a controlled, pH‑sensitive manner.
- In animal wound‑healing studies, the GHK‑Cu‑loaded polymer improved closure rates, tissue quality, and showed no toxicity.
Practical Outcomes
- For biohackers interested in GHK‑Cu for skin repair, this work suggests that a slow‑release delivery system could boost its effectiveness. While the study is pre‑clinical, it supports the idea of using gels or dressings that keep GHK‑Cu at the wound site longer, rather than single‑dose applications.
Summary
Researchers made a special water‑absorbing material that can hold and slowly release the anti‑aging peptide GHK‑Cu. They showed the material is safe for blood, releases the peptide steadily, and helps wounds close faster in animal tests compared to the material without the peptide.
Abstract
The main aim of this study was to evaluate the in vitro and in vivo efficiency of the polyaspartic acid- and acrylic acid-based superabsorbent polymer. The synthesized polymer was first investigated to check the blood compatibility by protein adsorption and blood clotting tests. Further, the GHK-Cu peptide was incorporated within the polymer and release studies were performed to evaluate the drug-delivery efficiency of the superabsorbent polymer. The polymer with best peptide release results were further used for in vivo analysis for wound healing. The healing efficiency of polymer with and without peptide was analyzed using wound closure, biochemical assay, histopathological, and toxicity studies.
Study Information
pubmed
2019
2019-11-19T00:00:00.000Z
10.1021/acsomega.9b00655
10
48