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GHRP-6

Growth Hormone Releasing Peptide-6, Growth hormone-releasing hexapeptide, His-D-Trp-Ala-Trp-D-Phe-Lys-NH2

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Studies 702

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Overview Studies Clinical Trials

Score 4

2008 pubmed

Fast conventional Fmoc solid-phase peptide synthesis with HCTU.

Hood. Christina A CA; Fuentes. German G; Patel. Hirendra H; Page. Karen K; Menakuru. Mahendra M; Park. Jae H JH

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Key Findings

HCTU is safe to handle (non‑toxic, non‑irritating, non‑corrosive) and inexpensive.
Using HCTU, coupling reactions finish in ≤5 minutes and deprotection steps in ≤3 minutes.
Total synthesis time for peptides (including GHRP‑6) was reduced by as much as 42.5 hours (≈1.8 days) while maintaining crude purity.
The method works for a range of biologically active peptides, not just GHRP‑6.

Practical Outcomes

If you have a basic solid‑phase peptide synthesizer, switch to HCTU as your coupling reagent to dramatically speed up production and lower costs. The faster cycle times mean you can produce more batches of GHRP‑6 or other peptides in a week, making self‑supply more feasible. Because HCTU is non‑toxic, handling safety is improved compared to many traditional reagents.

Summary

Researchers showed that a cheap, non‑toxic chemical called HCTU can make the lab process for building peptides like GHRP‑6 much faster. By swapping in HCTU, the steps that normally take many minutes can be done in under five minutes, cutting total production time by up to two days without lowering the quality of the peptide.

Abstract

1H-Benzotriazolium 1-[bis(dimethyl-amino)methylene]-5-chloro-hexafluorophosphate (1-),3-oxide (HCTU) is a nontoxic, nonirritating and noncorrosive coupling reagent. Seven biologically active peptides (GHRP-6, (65-74)ACP, oxytocin, G-LHRH, C-peptide, hAmylin(1-37), and beta-amyloid(1-42)) were synthesized with reaction times reduced to deprotection times of 3 min or less and coupling times of 5 min or less using HCTU as the coupling reagent. Expensive coupling reagents or special techniques were not used. Total peptide synthesis times were dramatically reduced by as much as 42.5 h (1.8 days) without reducing the crude peptide purities. It was shown that HCTU can be used as an affordable, efficient coupling reagent for fast Fmoc solid-phase peptide synthesis.

Study Information

Provider

pubmed

Year

2008

DOI

10.1002/psc.921