GHRP-6
Growth Hormone Releasing Peptide-6, Growth hormone-releasing hexapeptide, His-D-Trp-Ala-Trp-D-Phe-Lys-NH2
Effect of inhibitor and activator of ghrelin receptor (GHS-R1a) on porcine ovarian granulosa cell functions.
Sirotkin. Alexander V AV; Meszarošová. Monika M; Grossmann. Roland R; Benčo. Andrej A; Valenzuela. Francisco F
Key Findings
- (D‑Lys3‑GHRP‑6) increased markers of cell proliferation and reduced markers of apoptosis in porcine granulosa cells.
- (D‑Lys3‑GHRP‑6) raised the release of progesterone, testosterone, and estradiol from these cells.
- Both the ghrelin blocker and the ghrelin activator produced similar effects, suggesting the actions are not mediated by the GHS‑R1a receptor.
Practical Outcomes
- For most biohackers focused on longevity, metabolism, or performance, this study offers little direct, actionable guidance. It’s an early‑stage, in‑vitro finding about ovarian cell biology that would need extensive human research before any real‑world protocol could be considered.
Summary
A study on pig ovarian cells found that both a ghrelin receptor blocker (D‑Lys3‑GHRP‑6) and a ghrelin activator (ghrelin‑1‑18) boost cell growth, stop cell death, and increase hormone production. Because the same effects happen with a blocker, the results likely aren’t due to the usual ghrelin receptor, and the findings are limited to lab dishes, not humans.
Abstract
It was previously shown, that ghrelin and its agonistic analogue, ghrelin 1-18, can be a stimulator of ovarian cell functions (promoter of proliferation, inhibitor of apoptosis and stimulator of hormones release). The aim of our studies was to compare the action of two ghrelin analogues - ghrelin 1-18, activator of ghrelin receptors (GHS-R1a), and (D-Lys3)-GHRP-6, its inhibitor, on porcine ovarian granulosa cell functions. Effects of (D-Lys3)-GHRP-6 added at doses of 0, 1, 10 or 100 ng/ml on the expression of markers of proliferation (PCNA, cyclin B1, MAPK/ERK1,2), apoptosis (bax, p53, caspase 3) and release of steroid hormones (progesterone, testosterone, estradiol) were examined. In addition, some effect of ghrelin 1-8 on some of these parameters (expression of MAPK/ERK1,2, bax, p53) were verified. It was shown, that (D-Lys3)-GHRP-6 promotes all markers of granulosa cell proliferation, inhibits all markers of apoptosis and stimulates the release of all three steroid hormones. Similar effects of (D-Lys3)-GHRP-6 (inhibitor of GHS-R1a) and ghrelin 1-18 (its stimulator) suggest that the examined effects of these substances on porcine ovaries are not mediated by GHS-R1a. Both chemical analogues could be potentially useful for stimulation of reproductive processes, at least in in vitro conditions.
Study Information
pubmed
2011
2011-05-10T00:00:00.000Z
10.1016/j.ygcen.2011.05.001
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