The protective effects of humanin in rats with experimental myocardial infarction: The role of asprosin and spexin.
Onat. Elif E; Kocaman. Nevin N; Hancer. Serhat S
Key Findings
- Humanin pretreatment reduced serum markers of heart injury (AST, LDH, CK‑MB, Troponin I).
- Spexin levels rose after heart attack but fell when humanin was given; asprosin showed a similar pattern.
- Heart tissue showed less vein congestion, fewer ruptured blood cells, and fewer dead muscle fibers when humanin was administered before the attack.
Practical Outcomes
- Humanin appears to have a protective effect against heart damage in rats, suggesting it could be explored for heart health. However, the study used an injectable dose (2 mg/kg) in animals, so it isn’t ready for self‑use or direct translation to humans. Biohackers should view this as early‑stage evidence that may inspire future supplements or therapies, not a current protocol.
Summary
In rats, giving the tiny protein humanin before a chemically‑induced heart attack lowered heart‑damage enzymes in the blood and improved tissue appearance, while also changing levels of two other small proteins, asprosin and spexin, that might help protect the heart.
Abstract
In this study, by applying humanin (HN) before myocardial infarction (MI) in rats, its protection in MI and the possible roles in asprosin and spexin were investigated. The rats were divided into 7 groups each with 6 rats (group I (control), group II (HN 48th hour), group III (HN 7th day), group IV (MI 48th hour), group V (MI 7th day), group VI (MI + HN 48th hour), group VII (MI + HN 7th day). To create MI, 200 mg/kg isoproterenol (ISO) was administered subcutaneously to the rats. 2 mg/kg HN was given intraperitoneally (ip) to rats alone and before MI. Molecular parameters asprosin and spexin were examined by immunohistochemical in heart tissue. Biochemical parameters (aspartate aminotransferase (AST), lactate dehydrogenase (LDH), creatine kinase MB (CK-MB), Troponin I) were studied in serum by enzyme-linked immunosorbent assay (ELISA) method. It was found in the study that the levels of spexin elevated especially towards the 7th day after MI and decreased more significantly towards the 7th day, after HN application before MI. Asprosin elevated significantly towards the 7th day after MI and decreased especially on the 7th day after HN application before MI. Also, serum AST, LDH, CK-MB, and Troponin I levels tended to decrease and a significant decrease was detected in congested veins in the heart tissue at the 48th hour of MI and erythrocyte extravasation, congested veins and necrotic muscle fibers at the 7th day of MI in rats given HN before MI. It was concluded that HN has a cardioprotective effect in MI and asprosin and spexin might mediate this effect.
Study Information
pubmed
2023
2023-07-26T00:00:00.000Z
10.1016/j.heliyon.2023.e18739
4
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