The Neurovascular Protective Effect of S14G-Humanin in a Murine MCAO Model and Brain Endothelial Cells.
Peng. Tao T; Wan. Wencui W; Wang. Jingtao J; Liu. Yu Y; Fu. Zhenqiang Z; Ma. Xingrong X; Li. Junmin J; Sun. Guifang G; Ji. Yangfei Y; Lu. Jingjing J; Lu. Hong H; Liu. Yufeng Y
Key Findings
- HNG reduced brain infarct size and inflammation in a mouse stroke model
- It lowered harmful cytokines (TNF‑α, IL‑1β, IL‑6, MCP‑1) and adhesion molecules (VCAM‑1, ICAM‑1) in brain tissue
- In cultured brain endothelial cells, HNG improved survival under oxygen‑deprivation, cut ROS, and reduced white‑blood‑cell attachment
- The protective effect involved inhibition of the NF‑κB signaling pathway
Practical Outcomes
- For now, this study shows that a humanin analogue can protect brain blood vessels in mice, hinting at possible future therapies for stroke or vascular brain injury. Biohackers should view it as early‑stage evidence; no human dosing or safety data exist, so it isn’t ready for personal use yet.
Summary
A special version of the peptide humanin (called S14G‑humanin or HNG) was tested in mice that had a stroke and in brain blood‑vessel cells. It lowered brain damage, cut down inflammation signals and protected the cells from oxygen loss, mainly by blocking a stress pathway called NF‑κB. The work is still in animals, so it’s not a ready‑to‑use treatment for people yet.
Abstract
Endothelial dysfunction is fundamental to ischemic stroke and brain injury. The humanin analogue S14G-humanin (HNG) has been shown to be a cytoprotective derivative. In this study, we investigated the neuroprotective effects of HNG in vivo and in vitro. In a murine middle cerebral artery occlusion (MCAO) stroke model, HNG ameliorates cerebral infarction and suppresses the production of TNF-α, IL-1β, IL-6 and MCP-1 cytokines. HNG inhibits the expression of vascular adhesion molecules such as VCAM-1 and ICAM-1 in the cortex tissue. In mouse brain endothelial cells bEnd.3, HNG protects cell survival under oxygen deprivation (OGD) conditions. HNG suppresses ROS production as well as that of the same panel of cytokines and vascular adhesion molecules induced by OGD. HNG also reduces the numbers of THP-1 cells attached to bEnd.3 by OGD. Mechanistically, we show that HNG exerts its effect via inhibition of the NF- κB pathway factor IKKα, activation of IκBα and accumulation of p65 in the nucleus. Our data conclude that S14G-humanin serves as a neuroprotective factor, especially in brain vascular disorders. © 2018 IUBMB Life, 70(7):691-699, 2018.
Study Information
pubmed
2018
2018-07-01T00:00:00.000Z
10.1002/iub.1869
22
26