High-yield, solid-phase synthesis of humanin, an Alzheimer's disease associated, novel 24-mer peptide which contains a difficult sequence.
Evangelou. Alexandra A; Zikos. Christos C; Livaniou. Evangelia E; Evangelatos. Gregory P GP
Key Findings
- Humanin can be produced by Fmoc solidâphase synthesis on a 2âClâtritylamidomethyl polystyrene resin
- The segment R4G5F6S7C8L9 is a âdifficult sequenceâ that requires longer deprotection steps
- The optimized protocol yields a high overall amount of pure humanin
Practical Outcomes
- If you have chemistry skills and the right equipment, you can synthesize humanin yourself instead of buying it, giving you more control over purity and supply. However, the process is still technically demanding and not a simple atâhome hack.
Summary
Scientists figured out a reliable way to make the 24âaminoâacid peptide humanin in the lab using solidâphase chemistry, even though part of its sequence is tricky to handle. This method gives a good amount of pure peptide, which is needed for any further testing or use.
Abstract
Humanin is a novel, 24-mer residue bioactive peptide, which antagonizes Alzheimer's disease (AD) related neurotoxicity and offers a hope for developing new therapeutics against AD. Access to adequate amounts of pure humanin is a prerequisite for further, thorough, investigation of the pharmacological properties and therapeutic potency of the peptide. Until now, humanin has been obtained mainly by molecular biology techniques. In this work the Fmoc solid-phase synthesis of humanin on an in-house prepared 2-Cl-tritylamidomethyl polystyrene resin is described fully. Special precautions, i.e. prolonged deprotection steps, should be taken to achieve a high overall yield, since humanin seems to contain a 'difficult sequence' (R4G5F6S7C8L9) near its highly lipophilic, biologically important region L9L10L11L12.
Study Information
pubmed
2004
2004-10-01T00:00:00.000Z
10.1002/psc.572
11
21