Completed
NA
INTERVENTIONAL
NCT02877992
Tachykinin and Kisspeptin Expression in Human Granulosa and Cumulus Cells
View on ClinicalTrials.gov
Updated Dec 15, 2025
Brief Summary
The purpose of this project is to study the presence of expression differences - at RNA and protein level - of different members of the tachykinin family and kisspeptin and their receptors, between fertile women and infertile patients with different etiologies.
Interventions
Name:
Ovarian puncture
Type:
PROCEDURE
Description:
Donors and patients are subjected to ovarian puncture as part of their donation procedure or IVF treatment. Oocyte are retrieved in this procedure along with granulosa and cumulus cells. Donors and patients sign an informed consent in order to donate their granulosa and cumulus cells for the study.
Primary Outcomes
Measure:
Expression level of Substance P (SP)
TimeFrame:
4 years
Description:
Expression analysis at mRNA (mRNA = messenger ribonucleic acid) and protein level using techniques of real time quantitative PCR (PCR = polymerase chain reaction), immunocytochemistry and western blot
Measure:
Expression level of neurokinin A (NKA)
TimeFrame:
4 years
Description:
Expression analysis at mRNA and protein level using techniques of real time quantitative PCR, immunocytochemistry and western blot
Measure:
Expression level of neurokinin B (NKB)
TimeFrame:
4 years
Description:
Expression analysis at mRNA and protein level using techniques of real time quantitative PCR, immunocytochemistry and western blot
Measure:
Expression level of hemokinin 1 (HK-1)
TimeFrame:
4 years
Description:
Expression analysis at mRNA and protein level using techniques of real time quantitative PCR, immunocytochemistry and western blot
Measure:
Expression level of neurokinin 1 receptor (NK1R)
TimeFrame:
4 years
Description:
Expression analysis at mRNA and protein level using techniques of real time quantitative PCR, immunocytochemistry and western blot
Measure:
Expression of neurokinin 2 receptor (NK2R)
TimeFrame:
4 years
Description:
Expression analysis at mRNA and protein level using techniques of real time quantitative PCR, immunocytochemistry and western blot
Measure:
Expression level of neurokinin 3 receptor (NK3R)
TimeFrame:
4 years
Description:
Expression analysis at mRNA and protein level using techniques of real time quantitative PCR, immunocytochemistry and western blot
Measure:
Expression level of kisspeptin 1 (KISS1)
TimeFrame:
4 years
Description:
Expression analysis at mRNA and protein level using techniques of real time quantitative PCR, immunocytochemistry and western blot
Measure:
Expression level of kisspeptin 1 receptor (KISS1R)
TimeFrame:
4 years
Description:
Expression analysis at mRNA and protein level using techniques of real time quantitative PCR, immunocytochemistry and western blot
Trial Information
NCT ID
NCT02877992
Status
Completed
Study Type
INTERVENTIONAL
Phases
NA
Sponsor
Vida Recoletas Sevilla
Last Updated
December 15, 2025