KISS-1 knockdown inhibits cell growth, migration, and invasion in HTR-8/SVneo cells by regulating the GRP54-mediated PI3K/AKT signaling pathway.
Chen. Lingna L; Ruan. Yuying Y; Ni. Liping L; Wang. Guiting G; Gao. Yajuan Y; Zhang. Jindi J; Li. Dingheng D; Xu. Haiou H
Key Findings
- KISS-1 levels are lower in placental tissue from women with recurrent miscarriages.
- Increasing KISS-1 in trophoblast cells reduces their proliferation, migration, and invasion.
- The effect of KISS-1 depends on its receptor GPR54 and involves shutting down the PI3K/AKT signaling pathway.
Practical Outcomes
- For the biohacking community, this work does not provide actionable guidance on supplementation, dosing, or protocols for longevity, metabolic health, or performance. It is specific to reproductive health and not directly applicable to mainstream health optimization.
Summary
This study looked at a protein called kisspeptin (KISS-1) in cells that line the placenta and found that changing its levels can affect how those cells grow and move. The research is focused on why some women have repeated miscarriages, not on general health, fitness, or aging.
Abstract
Recurrent spontaneous abortions (RSA) affect reproductive health and increase the risk of subsequent abortions. To investigate the role of KISS-1/GPR-54 signaling in RSA progression. Villus tissue was collected from RSA patients, and human trophoblastic HTR-8/SVneo cells were used. KISS-1 and GRP54 levels were detected using RT-qPCR and immunohistochemistry. Western blotting was performed to analyze ZO-1 and ZEB1 levels. Cell proliferation was determined <i>via</i> CCK-8 and cell clone formation assays. Transwell assays were performed to assess cell migration and invasion abilities. KISS-1 was down-regulated in the villus tissues of RSA patients. KISS-1 overexpression dramatically inhibited trophoblast proliferation, migration, and invasion. Mechanistically, ZEB1 expression was down-regulated, whereas ZO-1 expression was up-regulated, after KISS-1 overexpression. GPR54 silencing neutralized the effect of KISS-1 in HTR-8/SVneo cells. Additionally, KISS-1 overexpression inactivated the PI3K/AKT signaling pathway through GRP54. The KISS-1/GPR-54 signaling axis regulates RSA progression by regulating the PI3K/AKT signaling pathway.
Study Information
pubmed
2023
2023-12-28T00:00:00.000Z
10.1080/08916934.2023.2297564
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