Associations of the KiSS-1 and GPR54 genetic polymorphism with polycystic ovary syndrome in Yunnan, China.
Zhao. Ting T; Zhang. Qiong Q; Xiao. Xiao X; Tao. Xinghua X; Gao. Meixiu M; He. Wenli W; Wu. Xiaomei X; Yuan. Tao T
Key Findings
- PCOS patients showed higher kisspeptin, BMI, LH, LH‑FSH ratio, prolactin, and testosterone than controls
- Serum kisspeptin positively correlated with LH and testosterone levels
- Specific KiSS‑1 (rs4889) and GPR54 (rs5780218) genotypes were associated with higher estradiol, though overall SNP frequencies were similar
Practical Outcomes
- For biohackers, the data hint that kisspeptin could be a biomarker for hormonal imbalance in PCOS, but there’s no dosage or protocol to act on yet. Any attempts to modulate kisspeptin should be experimental and monitored, as the study only shows association, not causation.
Summary
The study found that women with PCOS have higher blood levels of the hormone‑like peptide kisspeptin, and these levels go up together with LH and testosterone. Certain gene variants in the kisspeptin pathway were linked to different estradiol levels, but overall the gene frequencies weren’t different from healthy women. This suggests kisspeptin may be involved in PCOS, but the research didn’t test any treatments.
Abstract
Polycystic ovary syndrome (PCOS) is a heterogeneous condition that increases the risk for serious health complications among women. Thus, in this study, we aimed to identify polymorphisms in the KiSS-1 and GPR54 genes and investigate the associations between KiSS-1, BMI, FSH, LH, and estradiol in Chinese women with PCOS. This study recruited women with PCOS (<i>n</i> = 60) and controls (<i>n</i> = 60). We collected blood samples and measured endocrine indicators (kisspeptin, FSH, LH-FSH ratio, estradiol, prolactin, and testosterone). PCR and Snapshots were performed to screen single nucleotide polymorphisms (SNPs) of the KiSS-1 and GPR54 genes. Compared with the controls, endocrine and metabolic disturbances in women with PCOS were reflected in significantly higher levels of BMI, LH, LH-FSH ratio, prolactin, and testosterone. Serum kisspeptin levels were significantly higher and positively correlated with LH and T levels (<i>p</i> < 0.05). No identified SNPs of KiSS-1 and GPR54 showed significant differences in frequency between patients and controls. The mutant G allele was highly predisposed to polymorphism in the PCOS GPR54 gene r10407968. We observed a significant difference in estradiol (<i>p</i> = 0.023, <i>p</i> = 0.012) after comparing the values of LH, FSH, estradiol, progesterone, and testosterone among the three genotypes of rs4889 and rs5780218. The KiSS-1 rs4889 CC genotype and rs5780218 AA genotype showed significantly higher estradiol levels compared with the other two genotypes. These results suggest that kisspeptin plays a potential role in the etiology of PCOS.
Study Information
pubmed
2022
2022-07-28T00:00:00.000Z
10.1080/09513590.2022.2104246
7
35