Kisspeptin-10 Maintains the Activation of the mTOR Signaling Pathway by Inhibiting SIRT6 to Promote the Synthesis of Milk in Bovine Mammary Epithelial Cells.
Cao. Yu Y; Hu. Guiqiu G; Zhang. Qing Q; Ma. Lijun L; Wang. Jiaxin J; Li. Wen W; Ge. Yusong Y; Cheng. Ji J; Yang. Zhanqing Z; Fu. Shoupeng S; Liu. Juxiong J
Key Findings
- 100 nM kisspeptin‑10 boosts milk protein and fat synthesis in bovine mammary epithelial cells
- Kisspeptin‑10 activates mTOR signaling by inhibiting SIRT6 expression
- Over‑expressing SIRT6 reverses the kisspeptin‑10‑induced activation of mTOR and reduces milk synthesis
Practical Outcomes
- The findings are specific to cow milk production and do not provide a usable protocol for human health, longevity, or performance. There is no dosage guidance for people, and the relevance to human mTOR or metabolic pathways remains speculative.
Summary
The study shows that a peptide called kisspeptin‑10 can increase milk protein and fat production in cow mammary cells by turning on the mTOR pathway and blocking a protein called SIRT6, but it was done only in isolated cow cells, not in people.
Abstract
Kisspeptin-10 (Kp-10) is a peptide hormone that regulates normal physiological processes. The mechanism of Kp-10 in milk synthesis is still unclear. Therefore, bovine mammary epithelial cells (BMECs) were used to study the mechanism by which Kp-10 affects milk synthesis in BMECs. The GPR54 inhibitor and SIRT6 overexpression plasmid and siRNA were used to study the mechanism of regulating milk protein and milk fat synthesis by Kp-10. The results showed that 100 nM Kp-10 increased milk synthesis in BMECs. SIRT6 overexpression could significantly reduce the milk protein and milk fat synthesis in BMECs. Moreover, overexpression of SIRT6 reversed the activation of the Kp-10-induced mTOR signaling pathway. Further analysis suggested that SIRT6 might regulate the signal transduction of mTOR at the transcriptional level. These results strongly suggested that Kp-10/GPR54 activated the mTOR signaling pathway by inhibiting SIRT6 expression and then increased the milk synthesis in BMECs.
Study Information
pubmed
2021
2021-04-05T00:00:00.000Z
10.1021/acs.jafc.0c07613
13
27