Kisspeptin leads to calcium signaling in cultured rat dorsal root ganglion neurons.
Kelestimur. Haluk H; Bulut. Ferah F; Canpolat. Sinan S; Ozcan. Mete M; Ayar. Ahmet A
Key Findings
- Kisspeptin-10 raises intracellular calcium levels in cultured rat dorsal root ganglion neurons.
- The calcium rise still occurs, though reduced, without extracellular calcium, suggesting release from internal stores.
- The calcium response is not blocked by a protein kinase C inhibitor, indicating a PKC‑independent mechanism.
Practical Outcomes
- The study shows kisspein may influence pain pathways via calcium signaling, but it provides no human dosage or protocol. For biohackers, it’s an interesting mechanistic clue but not ready for safe self‑application; further research is needed before any practical use.
Summary
Kisspeptin-10 can trigger a rise in calcium inside rat sensory nerve cells, even when outside calcium is removed, and this effect doesn’t rely on the usual PKC pathway, hinting it may play a role in pain signaling.
Abstract
Although kisspeptin and GPR54 have been reported to be expressed in the neurons of the dorsal root ganglion (DRG) of rats, and kisspeptin has been suggested to be involved in pain modulation in rodents, there is no study on the effects and mechanisms of kisspeptin on sensory neurons. Therefore, the aim of this study was to investigate the effects and mechanism of kisspeptin on intracellular free calcium levels in cultured rat DRG neurons. Bath application of kisspeptin-10 increased intracellular free calcium levels ([Ca2+]i). In the absence of extracellular calcium, the kisspeptin induced an attenuated but still significant increase in [Ca2+]i. [Ca2+]i responses persisted in the presence of protein kinase C (PKC) inhibitor. Data from this study revealed that kisspeptin-10 activates [Ca2+]i signaling independent of PKC in cultured rat sensory neurons suggesting that peripheral site is also involved in the pain modulating effect of kisspeptin.
Study Information
pubmed
2021
2021-03-01T00:00:00.000Z
10.4149/gpb_2020047
3
32