Impact of serum and follicular fluid kisspeptin and estradiol on oocyte maturity and endometrial thickness among unexplained infertile females during ICSI.
Rehman. Rehana R; Zafar. Amara A; Ali. Arzina Aziz AA; Baig. Mukhtiar M; Alam. Faiza F
Key Findings
- Serum and follicular fluid kisspeptin levels rise through stimulation stages 1‑3 and drop after egg retrieval.
- Follicular fluid kisspeptin is higher than serum levels and strongly correlates with estradiol levels.
- Higher follicular fluid kisspeptin is positively associated with oocyte maturity (r = 0.511) and endometrial thickness (r = 0.522).
Practical Outcomes
- For biohackers interested in fertility, the data suggest kisspeptin could be a useful biomarker of ovarian response and uterine readiness during assisted reproduction. However, the study does not provide dosing guidelines or a supplement protocol, so the immediate actionable step is limited to monitoring if you have access to clinical testing.
Summary
The study found that higher levels of the hormone kisspeptin in both blood and the fluid around eggs (follicular fluid) during IVF cycles were linked to more mature eggs, thicker uterine lining, and better chances of pregnancy in women with unexplained infertility.
Abstract
To relate serum and follicular fluid (FF) kisspeptin and estradiol levels in different stages of stimulation during Intracytoplasmic Sperm Injection (ICSI) with oocyte maturity and endometrial thickness among unexplained infertile females. This cross-sectional study was carried out at the Australian Concept Infertility Medical Centre from March 2017 till March 2018. Fifty unexplained infertile females, booked for ICSI, were included in the study. Serum kisspeptin and estradiol were estimated by Enzyme-Linked Immunosorbent Assay in all four stages; 1: follicular stimulation, 2: ovulation induction, 3: oocyte pickup, and 4: embryo transfer. FF was aspirated during oocyte retrieval (stage 3) for the analysis of KP and estradiol. Pregnancy outcomes were categorized as non-pregnant, preclinical abortion, and clinical pregnancy. The age of the study subjects was 32.04 ± 2.29 (Mean±SD) years, with mean BMI of 28.51 ± 4.15 (Mean±SD) kg/m2. Mean serum kisspeptin and estradiol levels increased in all subjects as the stimulation proceeded stages 1-3; however, the mean dropped after retrieval of the oocytes (stage 4). Out of 27 female subjects who completed the cycle, 17 remained non-pregnant, 4 had preclinical abortion, and 6 acquired clinical pregnancy. The FF kisspeptin concentration was significantly higher than serum concentrations and positively correlated with serum and FF estradiol concentrations. FF-kisspeptin correlated with serum kisspeptin in Stage 3 (r = 0.930, p<0.001), maturity of oocyte (r = 0.511, p = 0.006) and endometrial thickness (r = 0.522, p = 0.005). Kisspeptin in stage 3 was also found to correlate with endometrial thickness (r = 0.527, p = 0.005) and with estradiol (r = 0.624, p = 0.001) independently. Increase in serum and FF-kisspeptin and estradiol levels from stages 1 to 3, resulted in an optimum endometrial thickness, probability of fertilization of oocytes and chances of clinical pregnancy in Assisted Reproductive Techniques /ICSI cycles of unexplained infertile females.
Study Information
pubmed
2020
2020-10-28T00:00:00.000Z
10.1371/journal.pone.0239142
15
29