Expression of reproductive-related genes and changes in oocyte maturation of goldfish broodstock (Carassius auratus) following injection of different exogenous kisspeptins.
Valipour. AbdolMajid A; Heidari. Behrooz B; Vaziri. Hamidreza H; Asghari. S Mohsen SM
Key Findings
- Kisspeptin (both fish and human forms) raised expression of kiss1, Kissr, and Cyp19 genes in goldfish brain and ovary.
- Fish treated with kisspeptin had a higher number of mature oocytes compared to control or GnRH (Ovaprim) treated groups.
- Human kisspeptin alone or combined with fish kisspeptin was the most effective at promoting oocyte maturation.
Practical Outcomes
- For hobbyist biohackers, this study offers limited direct use because it was done in goldfish, not humans. It does suggest that kisspeptin can strongly activate the reproductive hormone axis across species, hinting that future human-focused research might explore kisspeptin for fertility or hormonal modulation, but no actionable protocol for people can be derived yet.
Summary
Injecting kisspeptin peptides into adult female goldfish boosted the activity of genes that control reproduction and led to more mature eggs, especially when using the human version of the peptide or a mix of fish and human peptides.
Abstract
Kisspeptin, upstream of the hypothalamic-pituitary-gonadal axis, play an essential role in the reproductive process. In the present study, the effect of different types of kisspeptin, including goldfish (Carassius auratus) kiss1 kisspeptin (Kiss1), human kisspeptin (Hkiss) and their combination (Kiss1+H) on the reproductive-related genes (kiss1, Kissr and Cyp19) of adult female goldfish was investigated in comparison with Ovaprim (a synthetic GnRH hormone). Kiss1 and Hkiss were synthesized using a solid-phase synthesis approach. Peptides were injected at a dose of 100 μg/kg body weight. The brain and ovarian tissues of samples were separated for histological studies 24 hr post-injection. The expression of the kiss1, Kissr and Cyp19 genes was measured by RT-PCR. The results showed a significant increase in expression of the reproductive-related genes. Histological analysis revealed higher number of mature oocytes in kisspeptin treated groups compare to other ones. In conclusion, Hkiss and Kiss1+H are the most effective peptides in oocyte maturation and expression of reproductive-related genes. In addition, it seems that kisspeptins in other domestic animals can be used to stimulate the hypothalamus-pituitary-gonadal axis.
Study Information
pubmed
2021
2021-08-06T00:00:00.000Z
10.1111/rda.13998
7
32