The kisspeptin derivative kissorphin reduces the acquisition, expression, and reinstatement of ethanol-induced conditioned place preference in rats.
Gibula-Tarlowska. Ewa E; Grochecki. Pawel P; Silberring. Jerzy J; Kotlinska. Jolanta H JH
Key Findings
- Kissorphin (KSO) administered intravenously at 1‑10 nmol reduced the acquisition of ethanol‑induced conditioned place preference in rats.
- Acute KSO dosing before testing blocked both the expression of an already learned alcohol reward and the reinstatement of that reward after extinction.
- KSO alone did not produce place preference, aversion, or changes in locomotor activity, indicating a specific anti‑reward effect without general sedation or motor impairment.
Practical Outcomes
- For biohackers interested in managing alcohol cravings or exploring anti‑addiction strategies, kissorphin shows promise as an anti‑opioid peptide that can dampen alcohol’s rewarding effects in animal models. However, it is not yet available for human use, and dosing, safety, and efficacy in people remain unknown, so it should be viewed as a research insight rather than a ready‑to‑apply protocol.
Summary
A new peptide called kissorphin, which is similar to kisspeptin-10, was given to rats and was found to block the rewarding feeling they get from alcohol. It stopped the rats from learning to like places associated with alcohol, reduced the feeling of reward when they were already conditioned, and prevented a relapse after a break. The peptide didn't cause any preference or aversion on its own and didn't affect the rats' movement or coordination.
Abstract
Research has shown that opioids are involved in the rewarding effects of ethanol. Neuropeptide FF (NPFF) has been described as an anti-opioid peptide because, in many cases, it inhibits opioid and ethanol effects in rodents. Kissorphin (KSO) is a new peptide derived from kisspeptin-10 with structural similarities to NPFF. This peptide possesses NPFF-like biological activity in vitro. The aim of the current study was to investigate whether KSO (Tyr-Asn-Trp-Asn-Ser-Phe-NH2) influences the acquisition, expression, and reinstatement of ethanol-induced conditioned place preference (ethanol-CPP) in rats. The ethanol-CPP was established (conditioning for 5 days) by intraperitoneal (i.p.) administration of ethanol (1 g/kg, 20%, w/v) using an unbiased procedure. After that, one group of rats was used in final post-conditioning testing (expression of CPP) and the other group received a priming injection of ethanol after 10 days of extinction (reinstatement of CPP). Our experiments showed that KSO, given intravenously (i.v.) at the doses of 1, 3, and 10 nmol before every ethanol administration, inhibited the acquisition and, given acutely before the post-conditioning test or before the priming dose of ethanol, inhibited the expression and reinstatement of ethanol-CPP, respectively, in a dose-dependent manner. KSO given by itself neither induced place preference nor aversion and did not alter locomotor activity and coordination of rats. These results suggest that KSO can alter rewarding/motivational effects of ethanol. These data suggest this peptide possesses an anti-opioid character.
Study Information
pubmed
2019
2019-04-11T00:00:00.000Z
10.1016/j.alcohol.2019.04.001
10
61