Effects of kisspeptin-10 on in vitro proliferation and kisspeptin receptor expression in primary epithelial cell cultures isolated from bovine placental cotyledons of fetuses at the first trimester of pregnancy.
Martino. N A NA; Rizzo. A A; Pizzi. F F; Dell'Aquila. M E ME; Sciorsci. R L RL
Key Findings
- Kisspeptin‑10 altered proliferation of bovine placental epithelial cells, showing inhibition in some cell lines and stimulation in others.
- The direction of the effect was linked to the level of Kiss‑1R (kisspeptin receptor) mRNA – low change meant inhibition, up‑regulation meant stimulation.
- Kisspeptin‑10 did not affect progesterone secretion at any tested concentration.
Practical Outcomes
- At this time there’s no actionable protocol for humans; the study only shows that kisspeptin can influence cell growth in a species‑specific, early‑pregnancy context. Biohackers should not change dosing or expect performance benefits based on these findings.
Summary
Researchers tested kisspeptin‑10 on cow placental cells grown in the lab and found it could either slow down or speed up cell growth, depending on how much of the kisspeptin receptor the cells had. It didn’t change progesterone levels. The work is basic and done in bovine tissue, so it doesn’t give clear guidance for human use.
Abstract
Kisspeptin (Kp) and Kiss-1 receptor (Kiss-1R) expressions have been reported to be in the placenta, and a possible involvement of the Kiss-1R/Kps system in regulating trophoblast invasion and proliferation has been hypothesized. The aim of the present study was to investigate whether Kiss-1R activation by kisspeptin-10 (Kp-10) could modulate in vitro proliferation and progesterone (P4) secretion of bovine primary placental cell lines isolated from cotyledons of fetuses in the first trimester of pregnancy. The involvement of Kiss-1R in the cell responses observed was also analyzed. Uteri from cows at the first trimester of pregnancy were obtained from local abattoirs. Fetal cotyledon fragments were digested with collagenase in low glucose Dulbecco's Modified Eagle's Medium and cell lines were isolated. After being characterized for epithelial polygonal morphology, the presence of binucleate cells, male gender, and the expression of cytokeratin and zona occludens 2, cell lines were cultured in a low glucose Dulbecco's Modified Eagle's Medium-based expansion medium in the presence of 0.01, 0.1, 1, and 10 μM Kp-10. Control cells were cultured in the absence of Kp-10. Cell population doubling time was evaluated for each culture passage (P) from P1 to P10. Cells were tested for Kiss-1R mRNA expression analysis by real-time reverse transcription-polymerase chain reaction, and culture media were analyzed for P4 concentration by radioimmunoassay. Kisspeptin-10 modulated in vitro proliferation of epithelial cell lines isolated from cotyledons recovered from bovine fetuses in the first trimester of pregnancy. Inhibitory (line A) or stimulatory (line B) effects of Kp-10 on cell proliferation were found in different cell lines and observed cell responses were found to be related to Kiss-1R mRNA levels. Inhibition of cell proliferation matched with not significant variation of Kiss-1R expression, whereas stimulation of cell proliferation was found to be related to Kiss-1R upregulation. In both cell lines, no effect of Kp-10 on P4 secretion was found at any tested concentration. These results lead to the conclusion that the Kiss-1R/Kps system is involved in the regulation of cell proliferation of bovine placental cotyledon cell lines isolated at the first trimester of pregnancy but, at this gestational stage, it may not be involved in modulating placental P4 secretion.
Study Information
pubmed
2014
2014-12-03T00:00:00.000Z
10.1016/j.theriogenology.2014.11.033
16
38