Effects of kisspeptin-10 on lipid metabolism in cultured chicken hepatocytes.
Wu. J J; Fu. W W; Huang. Y Y; Ni. Y Y
Key Findings
- 100 nM Kp‑10 increased triglyceride, HDL‑C, and LDL‑C levels in cultured chicken hepatocytes.
- Cell viability was maintained at 100 nM but slightly reduced at 1,000 nM.
- Several lipid‑metabolism genes were down‑regulated, yet SREBP‑1 protein was up‑regulated.
Practical Outcomes
- The study shows kisspeptin can directly stimulate fat synthesis in bird liver cells, but there is no evidence it works the same way in humans. No safe dosage or protocol for people is provided, so biohackers should treat this as an early‑stage finding that needs far more research before any real‑world use.
Summary
In a lab study using chicken liver cells, the peptide kisspeptin‑10 (Kp‑10) at a concentration of 100 nM boosted the cells' production of triglycerides and cholesterol‑carrying particles without killing the cells. It also changed the activity of several genes that control fat metabolism, although the protein that drives fat synthesis (SREBP‑1) went up even though its gene level went down.
Abstract
Our previous studies showed that kisspeptin-10 (Kp-10) injected in vivo can markedly increase lipid anabolism in liver of quails. In order to investigate the direct effect of Kp-10 on lipid metabolism of hepatocytes in birds, cells were separated from embryos livers and cultured in vitro with 0, 100 and 1,000 nM Kp-10, respectively. The results showed that after 24 h treatment, cells viability was not affected by 100 nM Kp-10, but showed a mild decrease with 1,000 nM Kp-10 compared to the control cells. Based on the results of the cell viability, 100 nM dosage of Kp-10 was selected for the further study and analysis. Compared with control cells, total cholesterol (Tch) contents in 100 nM treated cells were increased by 51.23%, but did not reach statistical significance, while the level of triglyceride (TG), high density of lipoprotein-cholesterol (HDL-C) and low density of lipoprotein-cholesterol (LDL-C) were significantly increased. Real-time PCR results showed that ApoVLDL-II mRNA expression had a tendency to increase, genes including sterol regulatory element-binding protein-1 (SREBP-1), acetyl coenzyme A carboxylase α (ACCα), carnitine palmitoyltransferase 1 (CPT1), 3-hydroxyl-3-methylglutaryl-coenzyme A reductases (HMGCR) and stearyl coenzyme A dehydrogenase-1 (SCD1) mRNA in hepatocytes were significantly down-regulated by 100 nM Kp-10. However, contrary to its gene expression, SREBP-1 protein expression was significantly up-regulated by 100 nM Kp-10. Some of the significant correlations in mRNA expression were found between genes encoding hepatic factors or enzymes involved in lipid metabolism in liver of birds. These results indicate that Kp-10 stimulates lipid synthesis directly in primary cultured hepatocytes of chickens.
Study Information
pubmed
2012
2012-08-22T00:00:00.000Z
10.5713/ajas.2012.12189
9
34