Quantitative reverse transcriptase polymerase chain reaction analysis for KiSS-1 and orphan G-protein-coupled receptor (hOT7T175) gene expression in hepatocellular carcinoma.
Ikeguchi. Masahide M; Hirooka. Yasuaki Y; Kaibara. Nobuaki N
Key Findings
- KiSS‑1 levels stay about the same in normal and cancerous liver tissue
- The receptor hOT7T175 is much higher in cancerous liver tissue than in normal tissue
- Tumors with high KiSS‑1 and hOT7T175 are usually more advanced and linked to poorer survival
Practical Outcomes
- For biohackers, this research suggests that boosting kisspeptin pathways could potentially support tumor growth, so there’s no clear benefit for longevity or performance. Until more is known, it’s best to avoid trying to manipulate kisspeptin‑10 for health purposes.
Summary
This study looked at liver cancer tissue and found that the kisspeptin gene (KiSS‑1) and its receptor (hOT7T175) are often higher in advanced tumors, which may help the cancer grow. It doesn’t give any advice on using kisspeptin‑10 for health or performance.
Abstract
KiSS-1 has been cloned as a human metastasis suppressor gene and an orphan G-protein-coupled receptor (hOT7T175) identified as the endogenous receptor of the KiSS-1 product. In the present study, we evaluated the clinical importance of KiSS-1 and hOT7T175 gene expression in hepatocellular carcinoma (HCC). The expression levels of KiSS-1, hOT7T175 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) messenger RNAs (mRNAs) were analyzed quantitatively by real-time reverse transcriptase polymerase chain reaction (RT-PCR) in 60 surgically resected HCCs. The KiSS-1/GAPDH and hOT7T175/GAPDH ratios of tumors were compared with clinicopathological findings. Loss of KiSS-1 mRNA expression was not detected in HCCs. The mean KiSS-1/GAPDH ratio did not change between non-cancerous cirrhotic livers and carcinomas. On the other hand, the average hOT7T175/GAPDH ratios increased from non-cancerous livers (0.08) to carcinomas (0.48). Overexpression of KiSS-1 and hOT7T175 genes was recognized in 6 tumors, which were in an advanced stage and showed poor survival. Overexpression of KiSS-1 and hOT7T175 genes was frequently observed and correlated with HCC progression; thus, the possibility that overexpressed KiSS-1 and hOT7T175 peptides mediate growth signals into cancer cells in HCCs is suggested.
Study Information
pubmed
2003
2003-07-25T00:00:00.000Z
10.1007/s00432-003-0469-z
81
16