In rats, injecting alpha‑melanocyte‑stimulating hormone (MSH) or its strong version NDP‑MSH straight into a brain area that controls temperature makes the animals get hotter. The effect grows with higher doses, and certain short pieces of the peptide (the 4‑10 and 7‑13 fragments) also raise temperature, while the 1‑7 piece does not.

The thermoregulatory effects of alpha-melanocyte stimulating hormone (MSH), its potent analog, [Nle4,D-Phe7]-alpha-MSH (NDP-MSH), and the 1-7, 4-10, and 7-13 amino acid fragments of NDP-MSH were examined by administering these substances to the anterior hypothalamic-preoptic area (AHPOA) of rats. In Experiments 1a (MSH) and 1b (NDP-MSH), animals received 0, 0.5, 1, 5, 10, or 50 pM peptide in 0.5 microliters sterile saline (n = 6/group), with core rectal temperatures being recorded 0, 10, 20, 30, 40, 50, and 60 min after injection. In Experiment 2, subjects received 5 pM NDP-MSH1-7, NDP-MSH4-10, NDP-MSH7-13, NDP-MSH, or the vehicle, 0.5 microliters sterile saline, in a counterbalanced fashion (n = 13). Results indicated a significant effect of dose for both MSH, F(5, 30) = 2.81, p = 0.03, and NDP-MSH, F(5, 30) = 4.98, p = 0.002. A Newman-Keul's analysis indicated that mean temperatures for all groups receiving MSH or NDP-MSH were significantly greater than for the group that received saline (p < 0.05). An analysis of the data from Experiment 2 indicated a significant effect of substance, F(4, 48) = 17.31, p < 0.001. Mean temperature of animals receiving NDP-MSH, the 4-10, or the 7-13 fragments, did not differ from each other but were significantly greater than mean temperatures for animals receiving sterile saline or the 1-7 fragment of NDP-MSH (p < 0.05).