The study shows that a barnacle releases a mix of two chemicals, palmitic acid and a phospholipid, which together stop mussels from sticking to surfaces by interfering with the proteins they use to attach. This is a marine ecology finding and does not relate to human health or the peptide palmitoyl‑dipeptide‑6.

Chemical warfare among marine sessile organisms remains poorly understood. Chemical defense in calcareous-shelled organisms in particular has been largely neglected, yet this may be important in spatial dominance of crowded intertidal ecosystems. Using field survey data, spatial competition in intertidal zones between two calcareous-shelled sessile species are discovered, the barnacle Balanus albicostatus and the mussel Vignadula atrata. Using chemical analysis and bioassays, it is found that B. albicostatus releases chemical cues with inhibitory activity against the attachment of V. atrata. This allelochemical is identified as a blend of palmitic acid (PA) and 1-palmitoyl-sn-glycero-3-phosphocholine (PGPC) in a synergistic and unique ratio (1:1.92). This mixture of PA and PGPC synergistically reduced byssus thread production, adhesive plaque area and adhesion force of mussel foot proteins (MFPs) in V. atrata. Further analysis showed that this mixture down-regulated expression of the genes associated with byssus formation and adhesion (PreCol-NG, MFP2, MFP11, Tyr, BPP4, and PPO) and led to a lower activity of the enzyme polyphenol oxidase essential to mussel attachment, implying an underlying mechanism by which allelochemicals inhibit mussel attachment. This underlines the importance of allelopathy in interspecies competition between calcareous-shelled sessile organisms and provides information which may be useful for developing novel biofouling control systems.