The paper reviews how a special fat found in breast milk, called OPO, can be made using biotech methods and discusses safety and regulatory issues. It does not give any tips or protocols that hobbyist health‑optimizers could use for themselves.

1,3-dioleoyl-2-palmitoyl-glycerol (OPO), a functional lipid found in breast milk that is an essential nutritional additive in infant formula and holds significant dietary value for infants. Although conventional methods are widely applied to synthesize OPO, some drawbacks still need to be improved. Biosynthesis offers significant advantages over traditional methods for preparing human milk fat substitutes (HMFs), including OPO. However, the application of biosynthesis for lipid preparation is often constrained by food standards in various countries. Consequently, there is an urgent need to develop and update the standards for HMFs. This review provides a comprehensive summary of the clinical value, advantages, and disadvantages of the various methods used to prepare OPO. We further highlight that OPO synthesized through biotechnology is considered safe for infants when assessed for safety. Additionally, food safety standards should be updated regularly to ensure that more biotechnologically prepared functional lipids are accessible for human beings.