The study looked at how different fats are arranged inside a type of micro‑algae called Vischeria punctata. It found that most of the fat molecules have palmitic acid in the middle (sn‑2) position and palmitoleic acid on the outer positions (sn‑1 and sn‑3). This pattern could make the algae a good source of certain healthy fats, but the research does not relate to the peptide palmitoyl‑dipeptide‑6 or give any direct advice for health‑hacking protocols.

The positional distribution of fatty acids (FAs) in Vischeria punctata strain IPPAS H-242 during nitrogen starvation was analysed, and 22 individual FAs were identified in total lipids, TAGs, and native sn-1,2-diacylglycerols, comprising both saturated and polyunsaturated FAs. The predominant FAs were 14:0, 16:0, 16:1n-7, 18:1n-9, and 20:5n-3, accounting for more than 98.5% of the total FAs. TAGs demonstrated unique regioselectivity, with stearic, oleic, and eicosapentaenoic acids acylating the molecules at sn-1 and sn-3 positions. Instead, the sn-2 position was acylated with myristic, palmitic (8.4% and 40.1%, respectively) and palmitoleic (50.6%) acids, resulting in a total contribution of 99.1% from these three FAs. Palmitic, palmitoleic, and oleic acids made up 7.5, 73.9, and 13.1% of the sn-1(3) positions, respectively, or 94.5% in total. The molecular types of TAGs that accumulated in the cells of the microalga Vischeria punctata were as follows: UUU, 41.3%, and USU, 39.2%; TAGs of the UUS and USS types accounted for 9.4% and 9.0%, respectively. Tripalmitoleoyl-triacylglycerol, sn-1,3-dipalmitoleoyl-2-palmitoyl-glycerol, rac-1-oleoyl-2,3-dipalmitoleoyl-glycerol, and rac-1-oleoyl-2-palmitoyl-3-palmitoleoyl-glycerol were found as the most common molecular species of TAGs. Thus, Vischeria punctata strain IPPAS H-242 may be considered as a potential producer of structured TAGs with palmitic acid at the sn-2 position and palmitoleic acid at the sn-1 and sn-3 positions. The subsequent hydrolysis of such TAGs by pancreatic lipase produces palmitoleic acid and sn-2-palmitoylglycerol that have significant nutraceutical value and serve as a source of digestible palmitic acid for infant nutrition.