The temporary dynamics of inflammation-related genes expression under tuftsin analog Selank action.
Kolomin. Timur T; Morozova. Marina M; Volkova. Anastasiya A; Shadrina. Maria M; Andreeva. Lyudmila L; Slominsky. Petr P; Limborska. Svetlana S; Myasoedov. Nikolay N
Key Findings
- Selank caused a 3‑fold drop in C3 gene expression within 30 minutes.
- Both Selank and Gly‑Pro altered Casp1, Il2rg, and Xcr1 gene levels in a time‑dependent, wave‑like pattern.
Practical Outcomes
- For biohackers, this study hints that Selank may have short‑term immune‑modulating properties, but the work is limited to mice and uses intraperitoneal injection at a dose far from typical human use. Until human data are available, it’s not ready for direct self‑experimentation, though it may inform future research on oral or sub‑cutaneous formulations for anti‑inflammatory purposes.
Summary
A single injection of the peptide Selank (and its tiny piece Gly‑Pro) quickly lowered the activity of several genes that control inflammation in mouse spleen, showing a short‑term anti‑inflammatory effect.
Abstract
Previous studies have shown that synthetic tuftsin analogue Selank and its fragments cause a number of alterations in the expression of certain genes involved in inflammation in mouse spleen. In this work we studied the effect of Selank and its short fragment Gly-Pro on the temporary dynamics of C3, Casp1, Il2rg, and Xcr1 genes expression in mouse spleen after single intraperitoneal injection (100 μg/kg) of peptides using real-time PCR method. We found a significant 3-fold decrease in the C3 mRNA level just 30 min after Selank injection and similar alteration this gene mRNA level after Gly-Pro administration. A wave-like alteration in the Casp1 mRNA level was observed after Selank injection. We found a significant alteration in the mRNA level of the Il2rg gene at early time points after Selank and Gly-Pro administration and an almost equal reduction in the Xcr1 mRNA level 90 min after the administration of Selank and its fragment. Our results showed that, Selank and its short fragment Gly-Pro influence the expression of genes that mediate different types of immune responses, thereby maintaining the balance of the immune system. It should be noted that in most cases, there was a coincidence in the expression profiles of the studied genes after Selank and Gly-Pro administration. This might indicate an active contribution of the dipeptide to the final effect of Selank.
Study Information
pubmed
2013
2013-11-27T00:00:00.000Z
10.1016/j.molimm.2013.11.002
11
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