Studying the Toxic Effects of Some Biologically Active Peptides on the Model of Mouse Embryonic Stem Cells.
Kobylyanskii. A G AG; Zolotarev. Yu A YA; Andreeva. L A LA; Grivennikov. I A IA; Myasoedov. N F NF
Key Findings
- Semax (and some other peptides) increased survival of mouse embryonic stem cells under serum deprivation.
- The peptides showed little to no toxic effect on cell proliferation or neuronal differentiation at the tested concentrations.
- Selank, thyroliberin, and NGF reduced the proportion of GABA-positive neurons derived from stem cells.
Practical Outcomes
- For biohackers, this study mainly provides reassurance that Semax and similar peptides are unlikely to be harmful to early-stage cells, but it doesn't give direct guidance on dosing or performance benefits in humans. The findings are more about basic safety than actionable protocols for longevity or cognitive enhancement.
Summary
The study looked at how several peptide drugs, including Semax, affect mouse embryonic stem cells. It found that these peptides are not toxic to the cells, can help them survive when nutrients are low, and have only minor effects on turning the cells into neurons. The results suggest the peptides are safe in early development, but the work was done in mouse cells, not humans.
Abstract
We studied the effects of peptide drugs (HLDF-6, PGP, RPGP, and PGLP) and peptide pharmaceutical products (Semax, Selank, and thyroliberin) on proliferation and survival of mouse embryonic stem cells and their derivatives. Differentiation of mouse embryonic stem cells into neuronal precursors was evaluated. PGP and PGLP in concentrations of 10 and 0.1 μM, respectively, had little, but significant inhibitory effect on proliferative activity of cells. These peptides in concentrations of 10 and 0.1 μM, respectively, and Semax (10 and 0.1 μM) significantly increased the survival rate of mouse embryonic stem cells (serum deprivation). Moreover, study peptides had little effect on the formation of neuronal precursors from mouse embryonic stem cells. HLDF-6, Selank, and thyroliberin produced an insignificant effect on the differentiation of these cells into mature neurons. Analysis of differentiation of embryonic stem cells into GABA<sup>+</sup> neurons showed that Selank, thyroliberin (100 μM), and NGF (100 ng/ml) decrease the ratio of these cells by 61, 58, and 87%, respectively, in comparison with the control. Our results indicate that these peptide compounds do not produce toxic effect during the embryonic and fetal period of life.
Study Information
pubmed
2017
2017-10-24T00:00:00.000Z
10.1007/s10517-017-3891-y
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