A radioimmunoassay for African catfish growth hormone: validation and effects of substances modulating the release of growth hormone.
Lescroart. O O; Roelants. I I; Mikolajczyk. T T; Bosma. P T PT; Schulz. R W RW; Kühn. E R ER; Ollevier. F F
Key Findings
- A sensitive radioimmunoassay for catfish GH with detection limit 0.1 ng/ml
- Dopamine agonist apomorphine raises plasma GH in catfish in a dose‑dependent way
- A salmon GnRH analogue does not change GH levels in this species
Practical Outcomes
- There are no actionable protocols for human use. The findings are specific to fish physiology and don’t translate to sermorelin dosing or performance enhancement.
Summary
The paper describes a new test to measure growth hormone in African catfish and shows that dopamine drugs raise fish GH, while a salmon hormone analogue does not affect it. It doesn’t study sermorelin or human GH, so it offers no direct tips for people looking to boost their own hormones.
Abstract
A highly sensitive radioimmunoassay has been developed for measuring plasma growth hormone (GH) concentrations in the African catfish (Clarias gariepinus). The lower detection limit of the assay was 0.1 ng/ml and the standard curve had an ED50 value of 0.5 ng/ml. The validity of the assay was established and the effects of several neurotransmitters on the release of GH were examined. In vitro experiments, using a static culture system for dispersed pituitary cells, demonstrated that the GH release in African catfish was affected by growth hormone-releasing hormone and somatostatin. Single intraperitoneal injections with a dopamine agonist, apomorphine, produced significant and dose-dependent increases in plasma GH levels. Unlike carp, goldfish, and tilapia, a super-active analogue of salmon gonadotrophin-releasing hormone did not alter plasma GH levels in African catfish.
Study Information
pubmed
1996
10.1006/gcen.1996.0157