Adsorption effects of the doping relevant peptides Insulin Lispro, Synachten, TB-500 and GHRP 5.
Judák. Péter P; Van Eenoo. Peter P; Deventer. Koen K
Key Findings
- Peptides readily adsorb to glass and plastic surfaces, lowering the amount you can recover.
- Different peptides (including TB-500) behave differently on various consumables.
- Expensive low‑bind containers are not universally superior; selection should consider each peptide’s properties.
Practical Outcomes
- When you reconstitute or store TB-500, test how much you actually recover with your chosen tubes or vials. Choose containers that have been shown to minimize sticking for TB-500, or pre‑condition them, rather than assuming any low‑bind product will work best.
Summary
Peptides like TB-500 can stick to the walls of test tubes and bottles, which means you might lose some of the dose when you handle them. The study shows that not all fancy low‑bind containers work the same for every peptide, so you need to pick the right kind based on the peptide’s chemistry.
Abstract
The tendency of peptides to adsorb to surfaces can raise a concern in variety of analytical fields where the qualitative/quantitative measurement of low concentration analytes (ng/mL-pg/mL) is required. To demonstrate the importance of using the optimal glassware/plasticware, four doping relevant model peptides (GHRP 5, TB-500, Insulin Lispro, Synachten) were chosen and their recovery from various surfaces were evaluated. Our experiments showed that choosing expensive consumables with low-bind characteristics is not beneficial in all cases. A careful selection of the consumables based on the evaluation of the physico/chemical features of the peptide is recommended.
Study Information
pubmed
2017
2017-09-05T00:00:00.000Z
10.1016/j.ab.2017.09.003
12
16