Quantitative analysis of thymosin alpha1 in human serum by LC-MS/MS.
Tuthill. C W CW; Rudolph. A A; Li. Y Y; Tan. B B; Fitzgerald. T J TJ; Beck. S R SR; Li. Y X YX
Key Findings
- A sensitive LC‑MS/MS method was created to quantify thymosin‑alpha‑1 in human serum
- The assay can detect as low as 0.5 ng/mL and works up to 100 ng/mL
- Calibration curves showed excellent linearity (R² ≥ 0.9955) and good precision
Practical Outcomes
- This research provides a way to accurately measure thymosin‑alpha‑1 in blood, but it doesn’t offer dosing guidance, safety data, or performance benefits, so it has little direct use for biohackers looking for actionable protocols.
Summary
The paper only explains a lab technique for measuring thymosin‑alpha‑1 levels in blood, without any information on how to use the peptide for health or performance.
Abstract
A high performance liquid chromatography with tandem mass spectrometry (LC-MS/MS) method was developed to measure the thymosin alpha 1 (Talpha1) concentration in human serum. Tá1 in human serum was determined by solid phase extraction and reverse phase LC-MS/MS. The high-performance liquid chromatography (HPLC) system interfaced with the MS/MS system with a Turbo Ion spray interface. Positive ion detection and multiple reaction monitoring (MRM) mode were used for this human serum quantitation. Eight different concentration standards were used to establish the detection range. Six quality control (QC) and 2 matrix blanks were checked by calibration curves performed on the same day. The lower quantitation limit was 0.5 ng/mL Talpha1 in human serum. Calibration curves were established between 0.5 to 100 ng/mL by weighted linear regression. The correlation coefficients for different days were 0.9955 or greater. Quantitation of Talpha1 by the LC-MS/MS method is fast, accurate, and precise.
Study Information
pubmed
2000
2000-05-14T00:00:00.000Z
10.1208/pt010211
16
46