MicroELISA method for measurement of human serum thymosin alpha 1.
Weller. F E FE; Mutchnick. M G MG; Keren. D F DF; Goldstein. A L AL; Naylor. P H PH
Key Findings
- MicroELISA can detect human serum thymosin‑alpha‑1 with high sensitivity
- The assay is highly reproducible
- Results match those from the older radioimmunoassay method
Practical Outcomes
- For most biohackers this isn’t a usable protocol unless you have access to a lab. It mainly shows that reliable blood‑level testing is possible, which could support future dosing studies.
Summary
The paper introduces a tiny lab test (microELISA) that can accurately measure the amount of thymosin‑alpha‑1 in a blood sample, but it doesn’t give any advice on how to take the peptide or what effects to expect.
Abstract
A microELISA for the estimation of human serum thymosin alpha 1 is described. In this assay, antibody to thymosin alpha 1 is pre-incubated with the standard or serum at 4 degrees C. Unbound antibody in the liquid-phase then binds with solid-phase thymosin alpha 1. The method is sufficiently sensitive for measuring serum levels of thymosin alpha 1 and highly reproducible. The serum levels measured with the microELISA are comparable to serum levels of thymosin alpha 1 determined by the previously described radioimmunoassay for thymosin alpha 1.
Study Information
pubmed
1985
1985-06-12T00:00:00.000Z
10.1016/0022-1759(85)90163-2
17
10