Measurement of thymosin alpha 1 by disassociation microELISA.
Mutchnick. M G MG; Keren. D F DF; Weller. F E FE; McClure. J E JE; Goldstein. A L AL
Key Findings
- The assay can detect thymosin‑alpha‑1 at concentrations as low as 100 pg/ml
- It uses a competition format where liquid peptide competes with solid‑phase‑bound peptide for the antibody
- The method is reported to be specific, sensitive, and reproducible
Practical Outcomes
- For most biohackers this information isn’t directly usable because it requires lab equipment and expertise. It could be useful for those who want to verify how much thymosin‑alpha‑1 they have in a sample, but it doesn’t provide dosing guidance or health benefits.
Summary
The paper describes a lab test (microELISA) that can accurately measure very tiny amounts of the peptide thymosin‑alpha‑1, down to 100 pg/ml, using a specific rabbit antibody. It’s a technical method for researchers, not a guide for dosing or using the peptide in everyday health protocols.
Abstract
A disassociation microELISA was devised for the estimation of thymosin alpha 1, a chemically characterized thymic polypeptide isolated from bovine thymosin fraction 5. Antiserum to synthetic thymosin alpha 1 was raised in rabbits. Thymosin alpha 1 in liquid phase competed with a solid-phase-bound thymosin alpha 1 for this highly specific antibody. The method is specific, sensitive, reproducible and capable of detecting as little as 100 pg/ml of thymosin alpha 1.
Study Information
pubmed
1983
1983-05-27T00:00:00.000Z
10.1016/0022-1759(83)90334-4
4
25