[Effects of thymosin alpha1 on immune effector molecules of mouse].
Li. Wei-ye WY; Lu. Hui-min HM; Guo. Qiang Q; Hu. Wei-ming WM; Zhang. Zhao-da ZD
Key Findings
- CsA+Tα1 combo markedly reduced IL‑1α, IL‑2, IL‑6, IL‑17 and increased IL‑10 across 1‑21 days in mice
- Tα1 alone also lowered IL‑1α and IL‑6 and raised IL‑10 compared to control
- In vitro, Tα1‑treated lymphocyte cultures showed lower proliferation (OD) than controls
Practical Outcomes
- Tα1 may help shift the immune response toward an anti‑inflammatory profile, which could aid recovery or chronic inflammation management. For biohackers, start with clinically tested low doses (e.g., 1–2 mg subcut weekly) and monitor any changes in inflammation markers or well‑being. More human data are needed before high‑dose protocols.
Summary
In mice, thymosin‑alpha‑1 (Tα1) changed the immune signaling mix, lowering several pro‑inflammatory cytokines (like IL‑1α, IL‑2, IL‑6, IL‑17) and raising the anti‑inflammatory IL‑10, especially when given together with cyclosporine A. In cell cultures, Tα1 also reduced lymphocyte growth. This points to Tα1’s ability to push the immune system toward a calmer, Th2‑type state, which could be useful for people looking to manage inflammation or support immune balance, though the study is in mice and the doses are higher than typical human use.
Abstract
To analysis the effects of Talpha1 on the immune effector molecules in mouse immune system. Sixty five BABL/c mice were divided into four groups: CsA group (n=20), Talpha1 group (n= 20), CsA+Talpha1 group (n=20) and control group (n=5). In the 3 experimental groups, 10 mg/kg CsA, 400 microg/ kg Talpha1, 10 mg/kg CsA+400 microg/kg Talpha1 were respectively administrated by intraperitoneal injection daily. Luminex was performed for cytokine detection at 1 d, 7 d, 14 d, 21 d day after the above treatments. Lymphocyte culture was prepared with the mouse spleen suspension, and then treated with 0. 25 mg/mL CsA, 10 microg/mL Talpha1 or 0.25 mg/mL CsA+10 microg/mL Talpha1 in vitro, respectively. Three days later, OD values of each treated lymphocyte culture and several cytokines in the culture were measured. Compared with other groups, CsA+Talpha1 group had significant lower IL-1alpha, IL-2, IL-6, IL-17, and significant higher IL-10 at 1 d, 7 d, 14 d, 21 d after the treatments (P < 0.05). Three days after the culture, OD value in the control group was significantly higher than that in Talppha1 group, CsA group, and CsA+ Talpha1 group (P < 0.05). IL-1alpa and IL-6 in the control group were significantly higher than those in the experiment groups (P < 0.05), while IL-10 in the control group was significantly lower than that in the experiment groups (P < 0.05). IL-2 and IL-17 were similar. Talpha1 show regulatory effect on the immune effector molecules which could promote Th1 cells transforming to Th2 cells.
Study Information
pubmed
2014