[Anti-HBV effect of fusion protein (TA1-IFN) in vitro].
Lu. Nian-Fang NF; Huang. Ai-Long AL; Zheng. Rui-Qiang RQ; Zhu. Ya-Bin YB; Xia. Zhong-Fang ZF; Tang. Ni N; Yan. Ge G; Gao. Xiao-Ling XL; Wu. Ying Y
Key Findings
- TA1âIFN fusion protein showed doseâdependent inhibition of HBV antigens, reaching ~72% reduction in HBsAg and ~60% reduction in HBeAg at 8000âŻU/ml
- The fusion protein was less cytotoxic (â85% cell survival) than the combination of separate thymosinâalphaâ1 and interferonâalpha (â70% survival)
- Compared to the separate peptide combo, TA1âIFN achieved significantly higher antiviral effect and lower toxicity in vitro
Practical Outcomes
- The data hint that a single fused peptide could be more effective and safer for HBV than using thymosinâalphaâ1 and interferonâalpha together, but itâs only cellâculture evidence. No human dosing or safety info is available yet, so itâs not ready for selfâexperimentation. Keep an eye on clinical trials for TA1âIFN before considering it in personal health protocols.
Summary
In lab tests, a fused protein that links thymosinâalphaâ1 to interferonâalpha (TA1âIFN) shut down hepatitis B markers more strongly than taking the two peptides separately, and it was also gentler on the cells. The biggest effect was seen at the highest dose tested, cutting surface antigen levels by about 72% and eâantigen by about 60%, while keeping about 85% of the cells alive. The separate mix only cut the markers by roughly 40% and killed more cells.
Abstract
To investigate the anti-HBV effect of fusion protein thymosin alpha1-interferon alpha (TA1-IFN) in vitro and to compare its effect with a combination of interferon alpha and thymosin alpha1. After 2.2.15 cells were seeded for 24 hours, drugs of five serial concentrations (8000, 4000, 2000, 1000, 500 U/ml) were added to the wells, then the medium was changed every three days. After 2.2.15 cells were treated with drugs for 6 days, the medium was collected. The inhibitory rates on HBsAg and HBeAg were determined using Abbot kit, and the cytotoxicity of different drugs by means of MTT colorimetric assays was also observed. The inhibitory rate of fusion protein on HBsAg, HBeAg was dose-dependent and reached the maximum at 8000 U/ml concentration. In the meantime, the inhibitory rates of fusion protein on HBsAg and HBeAg were 72.2% +/- 0.8% and 60.4% +/- 1.1% respectively, and the cell survival rate was 85.2% +/- 2.0%; In the corresponding concentration, the inhibitory rates of combination thymosin alpha 1 and interferon alpha on HBsAg and HBeAg were 40.0% +/- 0.7%, 34.5% +/- 3.2% respectively. The results showed significant statistical differences between them; cell survival rate 70.0% +/- 1.9%, and the difference of the results was also significant. Cytotoxicity of fusion protein was weaker than a combination of thymosin alpha 1 and interferon alpha. Fusion protein TA1-IFN exerted stronger anti-HBV effects in vitro. Its anti-HBV effects in vitro were stronger than the combination of thymosin alpha and interferon alpha, and its cytotoxicity was weaker than the combination of thymosin alpha and interferon alpha. Our studies provided important evidence for clinical research on TA1-IFN, and also brought new hope for hepatitis B therapy.
Study Information
pubmed
2005