Enzyme immunoassay measurement of thymosin beta 4 in human serum.
Weller. F E FE; Mutchnick. M G MG; Goldstein. A L AL; Naylor. P H PH
Key Findings
- The enzyme immunoassay can detect as little as 2.5 ng/ml of thymosin beta‑4 in serum.
- No cross‑reactivity with other common serum proteins was observed.
- In 142 healthy adults, serum thymosin beta‑4 ranged from 3 to 82 ng/ml; newborn cord blood levels were lower.
Practical Outcomes
- For biohackers, the study mainly provides baseline blood levels of thymosin beta‑4, which could be useful for future monitoring but offers no actionable dosing or performance‑enhancing protocols for thymosin‑alpha‑1.
Summary
The paper describes a lab test that can accurately measure the amount of thymosin beta‑4 in human blood, showing normal adult levels between 3 and 82 ng/ml and lower levels in newborns, but it doesn’t give any advice on using thymosin‑alpha‑1 or how to boost health with these peptides.
Abstract
An enzyme immunoassay (EIA) for measurement of human serum thymosin beta 4 is described. Antiserum to synthetic thymosin beta 4, raised in rabbits, is incubated with a standard or serum. The unbound antibody in liquid-phase then binds with solid-phase thymosin beta 4. The EIA is both sensitive and accurate and is capable of detecting as little as 2.5 ng/ml thymosin beta 4 in serum. No cross-reactivity was observed with common serum proteins or other putative thymic hormones. High-performance liquid chromatography of serum samples reveals a single thymosin beta 4 peak that corresponds to the authentic thymosin beta 4 peak in the standard. Human serum levels range from 3 to 82 ng/ml in 142 healthy adult human volunteers. Newborn cord serum levels of thymosin beta 4 are lower than in adults.
Study Information
pubmed
1988