A practical synthesis of the 16C/15N-labelled tripeptide N-formyl-Met-Leu-Phe, useful as a reference in solid-state NMR spectroscopy.
Breitung. Sven T ST; Lopez. Jakob J JJ; Dürner. Gerd G; Glaubitz. Clemens C; Göbel. Michael W MW; Suhartono. Marcel M
Key Findings
- A mild solid‑phase synthesis followed by on‑bead formylation yields the labeled tripeptide.
- Typical batches produce more than 30 mg of the fully 13C/15N‑labeled peptide.
- The peptide crystallizes easily, making it a convenient standard for solid‑state NMR experiments.
Practical Outcomes
- For biohackers or self‑experimenters, this paper offers no direct health or performance benefit. It is only useful for labs that need a reliable NMR reference material.
Summary
The study shows a lab recipe for making a specially labeled three‑amino‑acid peptide that scientists use as a reference when they run solid‑state NMR scans. It’s a technical method for producing a research tool, not a health‑related supplement or therapy.
Abstract
A mild synthetic method for N-formyl-Met-Leu-Phe-OH (1) is described. After Fmoc solid phase peptide synthesis, on-bead formylation and HPLC purification, more than 30 mg of the fully (13)C/(15)N-labelled tripeptide 1 could be isolated in a typical batch. This peptide can be easily crystallised and is therefore well suited as a standard sample for setting up solid-state NMR experiments.
Study Information
pubmed
2008
2008-10-13T00:00:00.000Z
10.3762/bjoc.4.35
1
48