Development and Validation of an LC-MS/MS Method for Simultaneous Determination of Short Peptide-Based Drugs in Human Blood Plasma.
Fisher. Elizaveta N EN; Melnikov. Evgeny S ES; Gegeckori. Vladimir V; Potoldykova. Natalya V NV; Enikeev. Dmitry V DV; Pavlenko. Kirill A KA; Agatonovic-Kustrin. Snezana S; Morton. David W DW; Ramenskaya. Galina V GV
Key Findings
- A solid‑phase extraction LC‑MS/MS method was created to simultaneously quantify GnRH and somatostatin analogs in human plasma.
- The assay is linear from 0.5 to 20 ng/mL with correlation coefficients of 0.998‑0.999 for goserelin, buserelin, triptorelin, and octreotide.
- Accuracy and precision meet regulatory criteria (≤20% RSD at LLOQ, ≤15% at higher levels).
Practical Outcomes
- For most biohackers, this study doesn't change how you would take or dose triptorelin. It could be useful only if you have access to a specialized lab and want to monitor your own blood levels for research purposes. Otherwise, the findings are mainly of interest to scientists developing measurement techniques.
Summary
The paper describes a new lab test that can accurately measure tiny amounts of several peptide drugs, including triptorelin, in blood plasma. It shows the test works well over a range of concentrations and can be used in drug‑tracking studies, but it doesn't give any new advice on how to use the peptide for health or performance.
Abstract
A novel HPLC-ESI-MS/MS method for simultaneous gonadotropin-releasing hormone (GnRH) analogs and somatostatin analog quantitation was developed and validated. The developed method was successfully applied to pharmacokinetic studies. The sample preparation process included solid-phase extraction (SPE). Effective chromatographic separation of the analytes and internal standard (dalargin) was achieved with a C18 column, using a gradient elution with two mobile phases: 0.1% <i>v</i>/<i>v</i> formic acid (aqueous solution) and 0.1% <i>v</i>/<i>v</i> formic acid (acetonitrile solution). The linearity of the method was demonstrated within a concentration range of 0.5-20 ng/mL, with correlation coefficients between 0.998-0.999 for goserelin, buserelin, triptorelin, and octreotide, respectively. The relative standard deviation (RSD, %) values for method accuracy and precision did not exceed 20% at the lower level of quantitation (LLOQ) or 15% at other concentration levels.
Study Information
pubmed
2022
2022-11-14T00:00:00.000Z
10.3390/molecules27227831
9
41