EPIGENETIC MODIFICATION UNDER THE INFLUENCE OF PEPTIDE BIOREGULATORS ON THE "OLD" CHROMATIN.
Lezhava. T T; Jokhadze. T T; Monaselidze. J J; Buadze. T T; Gaiozishvili. M M; Sigua. T T; Khujadze. I I; Gogidze. K K; Mikaia. N N; Chigvinadze. N N
Key Findings
- Aging in elderly individuals is linked to increased heterochromatin (DNA condensation) that silences genes.
- Peptide bioregulators (Ala‑Glu‑Asp‑Gly, Lys‑Glu‑Asp‑Ala, Ala‑Glu‑Asp‑Pro, Lys‑Glu) selectively decondense heterochromatin in lymphocyte chromosomes, especially at ribosomal gene sites.
- The decondensation does not occur in pericentromeric structural heterochromatin, indicating region‑specific effects.
Practical Outcomes
- The results suggest these peptides can modulate chromatin structure, which might influence cellular aging processes, but the work is limited to lab‑based cell experiments. No dosage, safety, or real‑world protocol is provided, so biohackers should view this as early mechanistic insight rather than a ready‑to‑use supplement strategy.
Summary
The study looked at how short peptide mixes, including Cortagen, change the way DNA is packed in white blood cells from people aged 75‑88. Normally, aging makes DNA tighter (heterochromatin), which can turn off important genes. The peptides partially loosen this packing in specific chromosome regions, boosting activity of ribosomal genes, but they don’t affect all types of tightly packed DNA.
Abstract
In the present study, on the one hand, the epigenetic modification of condensed "old" chromatin was determined, and on the other hand, the influence of peptide bioregulators (Ala-Glu-Asp-Gly-Epitalon; Lys-Glu-Asp-Ala-Livagen; Ala-Glu-Asp-Pro - Cortagen and Lys-Glu - Vilon) on condensed chromatin in lymphocytes from old individuals. Were used molecular-cytogenetic methods: differential scanning calorimetry; activity of ribosomal genes of acrocentric chromosome satellite stalks-NORs; polymorphism of structural pericentromeric C-heterochromatin; variability of the facultative heterochromatin (sister chromatid exchanges - SCE) in the culture of lymphocytes from 75-88-year-old individuals. The analysis of results shows the chromosome progressive heterochromatinization (condensation of eu - and heterochromatin regions) occur in aging. Epigenetics process - heterochromatinization can deactivate many previously functioning active genes. It blocks certain stages of normal metabolic processes in the cell, which inhibits many specific enzymes and leads to aging pathologies. We show that peptide bioregulators induced unrolling deheterochromatinization (decondensation) of total heterochromatin, deheterochromatinization of satellite stalks of acrocentric chromosome, activating synthetic processes of ribosomal genes, does not cause deheterochromatinized of pericentromeric structural heterochromatin. This data also indicates that each of the studied peptide bioregulators (Ala-Glu-Asp-Gly; Lys-Glu-Asp-Ala; Ala-Glu-Asp-Pro and Lys-Glu) has a selective effect on definite regions of chromosomes. Thus, short peptide bioregulators induce selective heterochromatinization and deheterochromatinization of chromosome regions (remodeling of facultative heterochromatin) in individuals 75-88 years old that opens up new opportunities in the treatment of aging diseases.
Study Information
pubmed
2023